百合转P5CS-F129A基因的遗传稳定性和耐旱性检测

目的百合是世界上主要的商品花卉之一，具有很高的观赏价值和经济价值，然而目前的主栽东方百合品种耐旱和耐盐碱性较差，对其进行改良是提高百合价值的关键因素。方法对已获得的含抗逆相关基因P5CS-F129A的东方百合‘索邦’单拷贝转化株系L89和双拷贝株系L31进行了4年的组培继代培养和1年的温室栽培，检测了目的基因的稳定性和干旱胁迫处理下的表型和生理指标的变化。经Southern印迹杂交和cDNA检测显示目的基因能够稳定遗传。结果干旱胁迫处理结果表明:两个转基因株系叶片脯氨酸含量均高于普通‘索邦’，其中株系L31的脯氨酸水平最高；虽然干旱胁迫下两个转基因株系和普通‘索邦’叶片相对电导率均有所升高，但转基因株系叶片的相对电导率低于普通‘索邦’，重新灌水后，转基因株系恢复正常水平，普通‘索邦’未恢复；在表型性状等方面，经过胁迫并复水的株系L89和L31总体长势良好，两株系之间长势无显著差异，而普通‘索邦’叶片黄化严重，生长受阻。结论转P5CS-F129A基因植株具有更强的耐旱性和遗传稳定性，对抗逆性百合新品种的培育具有重要意义。 

Inheritance stability and drought stress test for P5CS-F129A transgenic lily

Objective] Lilium spp. is one of the main commercial flowers in the world which possesses both high ornamental and economic value. However, the current leading cultivars of Lilium Oriental hybrid are lack of drought and saline-alkaline tolerance thus it is crucial to improve the above properties. Method] Based on four-year subculture and one-year greenhouse culture, the stability of the target gene and changes of the phenotypes and physiological indices were tested for the single copy transformation line L89 and double copy line L31 with the P5CS-F129A gene of Lilium Oriental hybrid'Sorbonne'. The Southern blot and cDNA detection showed stable inheritance of the target gene. Result]The results of drought stress treatments demonstrated that the leaves of both transgenic lines contained higher proline content than the common ' Sorbonne', of which L31 contained the highest proline level. Though the relative leaf ion leakage of both transgenic lines and the common 'Sorbonne' rose in the drought stress process, the transgenic lines showed lower relative leaf ion leakage than the common 'Sorbonne'. After the second watering, the relative leaf ion leakage of both transgenic lines recovered to normal level, while the common 'Sorbonne' didn't. In the aspect of phenotype, both L89 and L31 grew well after the second watering, showing no obvious damage on growth, while the common'Sorbonne' showed severe leaf etiolation and growth retardation. Conclusion] The above research indicated that the P5CS-F129A transgenic gene lines possessed stronger drought tolerance which would be significant to the cultivation of new cultivars of stress-tolerant lily.