| 毛细管柱气相色谱法测定橡胶树胶乳中的乙烯含量及其变化 |
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| 引用本文： | 邓军,林秀琴,韦焕琦,林位夫.毛细管柱气相色谱法测定橡胶树胶乳中的乙烯含量及其变化[J].福建农林大学学报(自然科学版),2009,38(6). |
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| 作者姓名： | 邓军 林秀琴 韦焕琦 林位夫 |
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| 作者单位： | 1. 中国热带农业科学院橡胶研究所,海南,儋州,571737;云南省农业科学院甘蔗研究所,云南,开远,661600;云南省甘蔗遗传改良重点实验室,云南,开远,661600
2. 中国热带农业科学院橡胶研究所,海南,儋州,571737 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 采用毛细管柱气相色谱法测定橡胶树胶乳中的乙烯含量,并初步测定了乙烯刺激割胶不同割次的乙烯含量及其变化.该方法的线性范围为11.6-581.4μL.L-1,检测限为1.12μL.L-1,相对标准偏差(RSD)为1.01%.橡胶树采用乙烯利刺激(S/2 D/2+2.5%ET),涂药后第1-3割次的胶乳乙烯释放量分别为:151.6、31.1、17.5μL.L-1,而未经刺激的橡胶树(对照)乙烯含量低于检出限;第1-3割次的胶乳乙烯生成速率分别为:2.823、0.578、0.324 nL.g-1.h-1,表明经乙烯利刺激后,胶乳中的乙烯含量大量增加,生成速率发生了明显变化,均大于对照,且随割次的增多而降低.
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| 关 键 词： | 乙烯含量 橡胶树 气相色谱 胶乳 |
Determination of rubber latex ethylene content and its variation by capillary gas chromatography |
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| DENG Jun,LIN Xiu-qin,WEI Huan-qi,LIN Wei-fu.Determination of rubber latex ethylene content and its variation by capillary gas chromatography[J].Journal of Fujian Agricultural and Forestry University,2009,38(6). |
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| Authors: | DENG Jun LIN Xiu-qin WEI Huan-qi LIN Wei-fu |
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| Institution: | DENG Jun1,2,3,LIN Xiu-qin1,WEI Huan-qi1,LIN Wei-fu1(1.Rubber Research Institute,Chinese Academy of Tropical Agriculture Science,Danzhou,Hainan 571737,China,2.Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences,Kaiyuan,Yunnan 661600,3.Yunnan Key Lab of Sugarcane Genetic Improvement,China) |
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| Abstract: | A new determination method for ethylene content in latex of rubber tree (Hevea brasiliensis) by capillary gas chromatography (GC) was studied, and the ethylene content and its variation in latex were preliminarily determined from the different tapping under ethrel stimulation. It was showed that the RSD of ethylene in latex was 1. 01%, the detectable level of ethylene in latex was 1. 12 μL·L~(-1), and the linear range of ethylene in latex was 11. 6-581. 4 μL·L~(-1) by means of Shimadzu GC2010 GC. After treating with 2. 5% ethrel stimulant (S/2 D/2+2. 5% ET), the ethylene release rates of latex from the first to the third tapping were 151. 6, 31. 1, 17. 5 μL·L~(-1) respectively, and the ethylene production rates of latex from the first to the third tapping were 2. 823, 0. 578, 0. 324 nL·g~(-1)·h~(-1) respectively, but the ethylene release rate, which was lower than the detectable level, could not be detected in the control without stimulation. The experiment indicated that, after stimulating with ethrel, in latex there were significant changes in ethylene release rate and production rate, which were both higher than those of the control, and they obviously decreased as the increasing of tapping time. |
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| Keywords: | ethylene content rubber tree gas chromatography (GC) latex |
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