| 延边膜荚黄芪rDNA ITS区域克隆与序列分析 |
| |
| 引用本文： | 金钟范,王树斌,杨洪亮,邱菊,吴松权.延边膜荚黄芪rDNA ITS区域克隆与序列分析[J].延边大学农学学报,2012,34(1):27-29. |
| |
| 作者姓名： | 金钟范 王树斌 杨洪亮 邱菊 吴松权 |
| |
| 作者单位： | 1. 延边大学农学院,吉林延吉,133002
2. 吉林省大安市农业技术推广中心,吉林大安,131300 |
| |
| 基金项目： | 国家自然科学基金项目(30860036);吉林省科技厅项目(201115228);延边大学科技项目(延大科合字[2010]第14号) |
| |
| 摘 要： | 用PCR方法克隆延边地区膜荚黄芪的核糖体DNA ITS区域并进行序列分析。结果表明:延边地区膜荚黄芪ITS1的序列长度为228bp,5.8SrDNA长度为164bp,ITS2的序列长度为210bp;序列分析表明延边地区和甘肃的ITS1和5.8SrDNA完全一致,而ITS2第82位处有1个碱基的置换(T/C)。
|
| 关 键 词： | 膜荚黄芪 rDNA 内转录间隔区(ITS) |
Cloning and sequence analysis of rDNA ITS region of Yanbian Astragalus membranaceus |
| |
| JIN Zhong-fan,WANG Shu-bin,YANG Hong-liang,QIU Ju,WU Song-quan.Cloning and sequence analysis of rDNA ITS region of Yanbian Astragalus membranaceus[J].Journal of Agricultural Science Yanbian University,2012,34(1):27-29. |
| |
| Authors: | JIN Zhong-fan WANG Shu-bin YANG Hong-liang QIU Ju WU Song-quan |
| |
| Institution: | 1(1.Agricultural College of Yanbian University,Yanji Jilin 133002,China; 2.Agro-Tech Extension and Service Center of Daan City,Daan Jilin 131300,China) |
| |
| Abstract: | With the method of PCR and bioinformatics,rDNA ITS region of Astragalus membranaceus from Yanbian was cloned and analyzed.The results showed that the length of rDNA ITS1,5.8 S rDNA and rDNA ITS2 for A.membranaceus from Yanbian were 228 bp,164 bp and 210 bp respectively,no difference was observed in rDNA ITS1 and 5.8 S rDNA sequences,but one base inversion(T / C)was observed at the 82nd sequence in rDNA ITS2 between Yanbian and Gansu. |
| |
| Keywords: | Astragalus membranaceus rDNA ITS |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
