Cloning and Sequence Analysis of Actin Gene from Rehmannia glutinosa

Objective] The aim of this study is to clone and analyze the actin gene from Rehmannia glutinosa. Method] Degenerate primers were designed according to the conserved regions of actin sequences of Rehmannia glutinosa and its similar species,RT-PCR was next conducted to amplify the actin gene from Rehmannia glutinosa. Result] The amplified fragment is 724 bp and correspondingly 240 amino acids. The BLAST results indicate that the homology between the amplified fragment and other higher plants for actin gene sequences and amino acid are more than 80% and 90%,respectively,suggesting that the amplified fragment is the actin gene of Rehmannia glutinosa. Conclusion] Phylogenetic analysis shows that the actin gene of Rehmannia glutinosa has an intimate genetic relationship with actin7 gene of Nicotiana tabacum.