PCR法鉴定雏鸽性别的初步研究

雏鸽的雌雄外貌形态差别甚微,极难区别。为有效准确地鉴别雏鸽的性别,淘汰非目的性别,从而降低饲养成本,提高经济效益。本研究通过采用PCR方法,选定特殊的性别CHD1基因及鉴定引物,克隆出雏鸽特定性别基因序列鉴定雏鸽的雌雄。结果表明:在雏鸽雌性样本中扩增出1条带(777 bp),而雄性中未见扩增带,显示出CHD1基因的性别特异性,PCR扩增性别鉴定结果与样本解剖后对性腺观察的形态学鉴定结果也完全一致。在87份样品中,阳性克隆结果为25,阴性克隆结果为62,故87只雏鸽中雌鸽25只,雄鸽62只,其准确率达100%。

A Preliminary Study of Sex Identification of Young Pigeon with PCR Technique

Sex identification of young pigeon is difficult from appearance shape. In order to identify the sex of young pigeons to reduce feed cost and raise economic benefit, the PCR technique was used to amplify the CHD1 gene of pigeon. Results showed that a DNA fragment （777 bp） was amplified from female pigeons, none DNA fragment was amplified from the male. The resuhs indicated the CHD1 gene was sex specific. For the identification of male and female of the pigeon, the PCR amplification results and the gonad morphological dissection of samples were completely accords. Eighty-seven unknown sex samples were successfully identified （25 females and 62 males）. Sex identification by PCR technique was very importance for economic benefit in feeding pigeon.