微囊藻毒素的快速提取方法研究

我国水体蓝藻水华主要产生两种微囊藻毒素microcystin-LR(MCLR)和microcystin-RR(MCRR),针对蓝藻藻细胞内微囊藻毒素的提取和检测问题,提出微波和沸水浴取代传统溶剂萃取前处理的改善方法,采用标准品和实际样品分析了方法的可行性和提取效率.结果表明,沸水浴和微波(800W)处理10 min不会引起标准品的变异;以实验室培养的产毒铜绿微囊藻(Microcystis.aeruginosa)PCC7820为样品的提取实验表明,两种快速提取方法与传统的5%乙酸、70%甲醇萃取法提取效率的相对误差分别为4.6%(MCLR)和1.7%(MCRR).两种方法操作方便,并且可以有效地避免有机溶剂耗费和甲醇等有机溶剂残留对后续检测的干扰.应用该方法分析了天然水样,表明方法具有实用性.

Methods for rapid extraction of microcystin-LR and microcystin-RR

Simple analysis methods using microwave and boiling waterbath to solve the problem for microcystin-LR(MCLR) and microcystin-RR(MCRR) extraction from cyanobacteria cells were studied as a substitute for the traditional solvent extraction methods.Standards were used to confirm the feasibility and efficiency of both methods.Results showed that incubation in microwave(800W) and boiling waterbath for 10 minutes did not affect the structure of standards.And the efficiency of extraction from cultured toxic Microcystis.aeruginosa PCC7820 in both rapid methods were correlative to the classical 5% acetic acid and 70% methanol methods with S.D.of 4.6% for MCLR and 1.7% for MCRR.So both methods are convenient and reliable with the advantage of avoiding the expenditure of organic solvents and the interference with the subsequent biochemical detection from the residues of organic solvents.The method has been applied to analysis of field sample from a blooming pond.