构树上一种新的丁香假单胞菌致病变种的研究

 构树细菌性疫病是一种荧光假单胞菌引起的新病害。主要症状有叶片角斑,嫩梢肿大和幼枝溃疡。从江苏一带分离获得的12个构树菌株和3个桑树对比菌株进行交互接种试验,发现构树菌株与桑树菌株之间不能交互侵染其寄主。细菌学特征和LOPAT试验及其它37项生理生化和营养特性试验表明,两种菌的表型特征基本相似,仅在7种化合物的利用上存在差异。两种菌的血清学反应无相关性。细胞全蛋白SDS一聚丙烯酰胺凝胶电泳图谱也略有不同。试验结果证明,构树细菌性疫病细菌属于假单胞菌属(Pseudomonas),丁香假单胞菌(P.syringae)的一个新致病变种,定名为Pseudomonas syrzngae pv.broussonetiae pv.nov.

A NEW BACTERIAL DISEASE ON COMMON PAPERMULBERRY CAUSED BY PSEUDOMONAS SYRINGAE PV.BROUSSONETIAE PV. NOV.

Bacterial blight of common papermulberry (Broussonetiae papyrifera (L. ) Vent) is a new disease caused by plant pathogenic fluorescent Pseudomonas. Angular leafspot, stem canker and swollen shoot are the main symptoms of the disease. Twelve common paper-mulberry isolates were isolated from Nanjing, Suzhou, and.Yanzhou respectively. Three isolates of P seudomonas syringae pv. mori from mulberry were isolated in Suzhou and obtained from International Collection of Micro-organisms from Plants (ICMP).Cross inoculation tests with mulberry strains and common papermulberry strains showed that they couldn't infect each other. Comparative studies in bacteriological features, "LOPAT" tests, and other 37 physiological and biochemical tests indicated that the phynotypic characteristics of the two strains were similar. The protein pattern of SDS -PAGE had slight difference between them, but there was no relationship in serological reactions. Results indicated that common papermulberry strains were a new pathovar of Pseudomonas syringae, named as Pseudomonas syringae pv. broussonetiae pv. nov.