杂色鲍养殖环境中致病性弧菌分布调查

从深圳不同的杂色鲍人工养殖场采集并分类环境生物样品。应用TCBS平板分离并作生理生化初步鉴定弧菌,平板涂布法统计水样和各类生物每mL(或每g)所含弧菌总数,并应用基于16S~23SrDNA间区序列设计的4种水产病原弧菌特异性引物进行PCR,定性检测各类环境样品所携带致病性弧菌的分布状况。结果显示,弧菌广泛存在于杂色鲍养殖环境中,且杂色鲍养殖池池水中的弧菌密度大于进水口海水。在养殖环境生物中,不同环境生物中每克生物所携带的弧菌数差别很大,其中盘管虫、海蟑螂、等足类所携带的弧菌数最多,而海鞘携带的弧菌数最少。在常见的4种致病性弧菌的检测结果上,创伤弧菌和溶藻弧菌阳性率均为3.4%。通过研究弧菌在杂色鲍养殖环境中弧菌的分布特征,为杂色鲍养殖中流行性弧菌病的预防提供科学依据。

The investgation of Vibrio spp. in abalone culture pond

Environment organisms were collected from the culture ponds of Haliotis diversicolor , and had been classified and gathered respectively. Vibrios were selected by TCBS plate and identified by phenotypic characterizatioin. Counting method with coating on TCBS plate was applied to estimate the vibrios number in different sample. The PCR methods based on the 16S~23S rDNA sequences primers,were applied to detect 4 species aquatic pathogenic vibrios. There were vibrios allover the environment of abalone pond. And vibrios in pond water are much higher than that in influent. In aquaculture environment,it is quite different for the vibrios in per gram among all sorts of organisms. The Hydroides, Ligidaes and Isopods have the most vibrio quantity. However, the Ascidians has the minimal quantity. As a result of using 4 species pathogen probes,the positive rate of Vibrio alginolyticus and V. vulnificus is 3.4%. It can be provided references for preventing the epidemic diseases by studying the distribution of vibrios in environment of abalone culture pond.