枣树叶片愈伤组织培养与再生植株的研究

为探讨枣树组织培养育种及快繁技术,以木枣、骏枣和狗头枣组培苗叶片为外植体,通过诱导愈伤组织形成再分化出不定芽,从而建立高效的再生系统。试验结果表明,不同基因型或同一基因型的不同部位的材料其培养效果间有差异;试管枣苗茎尖倒数1～3片叶是合适的外植体材料;6-BA与2,4-D在枣树叶片愈伤组织诱导中有极显著的交互作用,二者的配比浓度水平影响愈伤组织的诱导增殖和质量;枣树叶片愈伤组织诱导和继代增殖培养适宜的培养基是3/4MS+6-BA0.2 mg.L-1+2,4-D 2.0 mg.L-1+琼脂0.55%+蔗糖3%。6-BAI、BA与TDZ在枣树愈伤组织分化培养中有显著的协同作用,不定芽诱导的适宜培养基是CYI+6-BA 1.0 mg.L-1+IBA 0.2 mg.L-1+TDZ 0.015 mg.L-1。

Callus culture from leaves and plant regeneration of jujube trees

In order to study the rapid propagation technique of Zizyphus jujuba by tissue culture,take the leaf of tissue culture seedlings of Chinese dates named as Muzao,Junzao and Goutouzao as explants.Through adventitious bud redifferentiated from the calli,a highly effective plant regeneration system was established.The results showed that explants of different genotype or different position of same genotype are different in cultured effects.The first to third leaves backwards from shoot-tip to down are suitable to be as explants.The 6-BA and 2,4-D have great significant interaction in callus induction and culture from jujube leaves.Their compounded ratio effected callus induction proliferation quantity and quality.The suitable medium is 3/4MS +0.2 mg·L-1 6-BA+2.0 mg·L-1 2,4D+0.55% agar+3% sucrose for callus induction and subculture from jujube leaves. The 6-BA,IBA with TDZ in callus differentiation culture of jujube trees showed they have significant effect in conjunction each other.The suitable medium is CY I+1.0 mg·L-1 6-BA+0.2 mg·L-1 IBA+0.015 mg·L-1 TDZ for inducing adventitious buds from jujube leaves.