LED不同光质对菘蓝愈伤组织诱导及再生的影响

目的]为研究不同光质对菘蓝愈伤组织诱导及再生的影响,方法]以定蓝1号菘蓝种子为材料,设置6个LED光照处理,分别为：荧光灯(CK),100R,100B,80R/20B,70R/30B,50R/50B,比较不同光质对菘蓝愈伤组织诱导、再生及生根情况,同时测定再生植株叶绿素含量及可溶性糖含量。结果]结果表明：红蓝混合光处理较单一红蓝光有明显优势,70R/30B处理下,菘蓝愈伤组织愈伤生长状态最好,表现为颜色绿，质地致密，分化率最高为68.75%,再生植株生根率最高为92.86%，生根状态表现为生根早，根粗壮，根长等特征，均显著高于对照。70R /30B 处理下,叶绿素a、总叶绿素含量及可溶性糖含量均达到最大值。结论]综上,红蓝混合光处理有利于菘蓝愈伤组织的诱导分化,再生植株的形态建成,叶绿素合成、可溶性糖积累,其中以70R/30B为最适光源。

Effects of Light Quality of Light Emitting Diode (LED) on Callus Induction and Regeneration of Indigoblue Woad

To study the effects of light quality of light emitting diode (LED) on callus induction and regeneration of indigoblue woad, the seeds of indigoblue woad (Isatis indigotica Fort)‘Dinglan1’were used as the material. Six light treatments were set as follows: fluorescent lamp (CK), 100R, 100B, 80R/20B, 70R/30B and 50R/50B. The contents of chlorophyll and soluble sugar were detected. The results showed that the complex light of the red and blue LED had better effect compared with only red or blue LED. Under 70R/30B treatment, the callus of indigoblue woad grew best. It was green and had close texture. The differentiation rate was 68.75%. The rooting rate of regenerated plants was 92.86%. The rooting status was characterized by early rooting, thick root and long root under 70R/30B, which were significantly higher than those of the control. Under 70R/30B treatment, the content of chlorophyll a, total chlorophyll and soluble sugar reached the maximum, 0.497 mg/g, 0.654 mg/g and 0.244 mg/g respectively. In a summary, it suggests that the complex light of the red and blue LED is suitable for the induction and differentiation of callus, morphogenesis of regeneration plant, chlorophyll synthesis and soluble sugar accumulation. 70R/30B is the best light.