| 与石刁柏性别基因M紧密连锁的AFLP新标记 |
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| 引用本文： | 韩莹琰,杨凯,范双喜,张振贤.与石刁柏性别基因M紧密连锁的AFLP新标记[J].园艺学报,2007,34(3):779-782. |
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| 作者姓名： | 韩莹琰 杨凯 范双喜 张振贤 |
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| 作者单位： | (北京农学院植物科学技术系, 北京102206; 中国农业大学农学与生物技术学院蔬菜系, 北京100094; 北京农学院农业应用新技术北京市重点实验室, 北京102206) |
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| 摘 要： | 运用BSA法构建石刁柏雌雄基因池, 用64种AFLP引物组合共检测到4个多态性位点, 单株验证结果表明, 引物组合E-ACG/M-CTT产生的多态性片段(命名为E-ACG/M-CTT2156) 与M 基因连锁,片段大小为156 bp; 连锁遗传距离为8.33 cM。
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| 关 键 词： | 石刁柏 性别基因M AFLP标记 BSA |
| 文章编号： | 0513-353X（2007）03-0779-04 |
| 修稿时间： | 2007-01-042007-04-18 |
AFLP Markers Linked with M Gene in Asparagus officinalis L. |
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| HAN Ying-yan,YANG Kai,FAN Shuang-xi,ZHANG Zhen-xian.AFLP Markers Linked with M Gene in Asparagus officinalis L.[J].Acta Horticulturae Sinica,2007,34(3):779-782. |
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| Authors: | HAN Ying-yan YANG Kai FAN Shuang-xi ZHANG Zhen-xian |
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| Institution: | (;1. Depatrment of Plant Science and Technology, Beijing Agricultural College, Beijing 102206, China;2. Department of Vegetable,College of Agriculture and Biotechnology, China Agricultural University, Beijing 100094, China;3. Key Laboratory of AgriculturalNew Technology and Application in Beijing, Beijing Agricultural College, Beijing 102206,China) |
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| Abstract: | In present study,the male and female bulk were set up by means of BSA,and AFLP was used to study the sex difference on Asparagus officinalis L.,4 polymorphic segments were detected with 64 AFLP selective primer pairs.The result of individual amplification showed that the specific 156 bp fragment from primer combination(E-ACG,M-CTT),named E-ACG/M-CTT-156,was linked with M gene at a linkage genetic distance of 8.33 cM. |
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| Keywords: | BSA |
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