Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair |
| |
Authors: | Mees Alexandra Klar Tobias Gnau Petra Hennecke Ulrich Eker Andre P M Carell Thomas Essen Lars-Oliver |
| |
Institution: | Department of Chemistry and Biochemistry, Butenandt-Strasse 5-13, Ludwig Maximilians University, D-81377 Munich, Germany. |
| |
Abstract: | DNA photolyases use light energy to repair DNA that comprises ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine dimers (CPDs). Here we report the crystal structure of a DNA photolyase bound to duplex DNA that is bent by 50 degrees and comprises a synthetic CPD lesion. This CPD lesion is flipped into the active site and split there into two thymines by synchrotron radiation at 100 K. Although photolyases catalyze blue light-driven CPD cleavage only above 200 K, this structure apparently mimics a structural substate during light-driven DNA repair in which back-flipping of the thymines into duplex DNA has not yet taken place. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|