| 大丽菊花瓣衰老过程中保护酶活性变化的研究 |
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| 引用本文： | 阚雪芹,孙悦,苏媚,周丽丽,徐文斐,谈建中.大丽菊花瓣衰老过程中保护酶活性变化的研究[J].北方园艺,2010(19):124-127. |
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| 作者姓名： | 阚雪芹 孙悦 苏媚 周丽丽 徐文斐 谈建中 |
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| 作者单位： | 苏州大学,建筑与城市环境学院园艺系,江苏,苏州,215123;苏州大学,建筑与城市环境学院园艺系,江苏,苏州,215123;苏州大学,建筑与城市环境学院园艺系,江苏,苏州,215123;苏州大学,建筑与城市环境学院园艺系,江苏,苏州,215123;苏州大学,建筑与城市环境学院园艺系,江苏,苏州,215123;苏州大学,建筑与城市环境学院园艺系,江苏,苏州,215123 |
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| 摘 要： | 为探讨观赏植物大丽菊衰老相关的生理生化指标,对不同开花时期花瓣的蛋白质表达量及保护酶活性进行了研究。结果表明:随着大丽菊花朵的开放与衰老,花瓣的可溶性蛋白质含量持续减少,而过氧化氢酶(CAT)的表达量呈先增后减的变化倾向。同时,过氧化氢酶与超氧化物歧化酶(SOD)活性都表现为先升后降的变化趋势,但二者在变化时序上存在差异,而过氧化物酶(POD)活性则表现为前期下降后期上升的倾向。由此推测SOD、CAT及POD可能依次是大丽菊花瓣衰老的不同阶段清除活性氧的关键酶。
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| 关 键 词： | 大丽菊 衰老 双向电泳 保护酶活性 过氧化氢酶 |
Analysis of Enzyme Activity of Protective System in Dahlia pinnata Cav Petals During Senescence |
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| KAN Xue-qin,SUN Yue,SU Mei,ZHOU Li-li,XU Wen-fei,TAN Jian-zhong.Analysis of Enzyme Activity of Protective System in Dahlia pinnata Cav Petals During Senescence[J].Northern Horticulture,2010(19):124-127. |
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| Authors: | KAN Xue-qin SUN Yue SU Mei ZHOU Li-li XU Wen-fei TAN Jian-zhong |
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| Institution: | KAN Xue-qin,SUN Yue,SU Mei,ZHOU Li-li,XU Wen-fei,TAN Jian-zhong(Department of Horticulture,School of Architecture and Urban Environment,Soochow University,Suzhou,Jiangsu 215123) |
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| Abstract: | To investigate the physiological-biochemical character related to the senescence of ornamental Dahlia pinnata Cav,the protein expression level and protective enzymes activity were analyzed by using the petals in different flowering periods.The results showed that the content of soluble protein decreased continuously,and the expression of CAT first increased and then decreased during the petal senescence.At the same time,the activity of CAT and SOD rose first and then fell,which changed in different periods.... |
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| Keywords: | Dahlia pinnata Cav senescence two dimensional electrophoresis protective enzyme activity catalase |
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