| 土壤磷酸酶测定中不同缓冲溶液对显色液吸收波长的影响 |
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| 引用本文： | 夏栋,许文年,赵自超,赵娟,祝顺波. 土壤磷酸酶测定中不同缓冲溶液对显色液吸收波长的影响[J]. 湖北农业科学, 2012, 0(5): 997-999 |
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| 作者姓名： | 夏栋 许文年 赵自超 赵娟 祝顺波 |
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| 作者单位： | 三峡大学三峡库区生态环境教育部工程研究中心 |
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| 基金项目： | 国家自然科学基金项目(50879043);国家水体污染控制与治理科技重大专项课题(2008ZX07104-003-03) |
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| 摘 要： | 确定了土壤磷酸酶测定时最大吸收波长以及显色时应加入的最佳pH缓冲溶液。研究结果表明,加入pH为8.0~10.0的缓冲液时显色时间快,在可见光区的最大吸收峰在604 nm处,其中以pH 9.0的吸光度最高,摩尔吸光系数为1.717×104L/(mol.cm),灵敏度较高。因此确定加入的最佳缓冲溶液pH为9.0,同时选择604 nm作为最大吸收波长。
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| 关 键 词： | 土壤磷酸酶 缓冲溶液 吸收波长 pH 测定 |
Effects of Different Buffer Solution on Absorption Wavelength in Determination of Soil Phosphatase |
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| XIA Dong,XU Wen-nian,ZHAO Zi-chao,ZHAO Juan,ZHU Shun-bo. Effects of Different Buffer Solution on Absorption Wavelength in Determination of Soil Phosphatase[J]. Hubei Agricultural Sciences, 2012, 0(5): 997-999 |
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| Authors: | XIA Dong XU Wen-nian ZHAO Zi-chao ZHAO Juan ZHU Shun-bo |
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| Affiliation: | (Engineering Research Center of Eco-environment in Three Gorges Reservoir Region,Ministry of Education,Three Gorges University, Yichang 443002,Hubei,China) |
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| Abstract: | The maximum absorption wavelength and the optimal pH buffer solution in determination of soil phosphatase had been determined.The results showed that the buffer pH in the range of 8.0～10.0 colored fast,and the maximum absorption peak was 604 nm.The absorbance of the solution added the buffer(pH=9.0) was the maximum,and the molar absorptivity was 1.717 × 104 L/(mol·cm).The suggestion was that the optimal pH buffer solution was 9.0,and 604 nm was chosen in determination of soil phosphatase. |
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| Keywords: | soil phosphatase buffer solution absorption wavelength pH determination |
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