| 药渣纤维素降解菌的筛选及酶活测定 |
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| 引用本文： | 郭建军,范汉东,黄晓萍,杨一兵,黄臣臣.药渣纤维素降解菌的筛选及酶活测定[J].湖北农业科学,2012(4):689-692. |
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| 作者姓名： | 郭建军 范汉东 黄晓萍 杨一兵 黄臣臣 |
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| 作者单位： | 江西省科学院微生物研究所;广东省茂名市第一中学 |
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| 基金项目： | 江西省科技厅项目(2011BDH80035,2010BNA08000);江西省科学院项目(2010-YGJ-01,2010-YGJ-02) |
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| 摘 要： | 以药渣和羧甲基纤维素钠(CMC-Na)为碳源,通过平板初筛及多种酶活综合测定复筛,从深层封土、牛粪堆肥等样品中筛选到8株纤维素降解菌,其中菌株JX9的酶活最高,酶系组成最合理,其Cx、Cb、C1和FPA分别达到82.41、5.71、15.52和7.64 U/mL。
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| 关 键 词： | 药渣 纤维素降解菌 筛选 纤维素酶 |
Screening and Cellulase Activity Determination of Herbal Residues Cellulose-decomposing Bacteria |
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| GUO Jian-jun,FAN Han-dong,HUANG Xiao-ping,YANG Yi-bing,HUANG Chen-chen.Screening and Cellulase Activity Determination of Herbal Residues Cellulose-decomposing Bacteria[J].Hubei Agricultural Sciences,2012(4):689-692. |
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| Authors: | GUO Jian-jun FAN Han-dong HUANG Xiao-ping YANG Yi-bing HUANG Chen-chen |
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| Institution: | 1.Institute of Microbiology,Jiangxi Academy of Sciences,Nanchang 330029,China; 2.First Middle School of Maoming City,Maoming 525000,Guangdong,China) |
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| Abstract: | Cellulose-degrading bacteria play an important role in degradation of cellulose material.Using the herbal residues or the carboxymethyl cellulose-Na(CMC-Na) as carbon resource,8 cellulose-degrading bacteria strains were isolated from rotten wood,herbal residues compost soil,forest defoliation soil and farmhouse compost by plate screen and cellulase activity determination.Strain JX9 had the maximum cellulase activity with an optimal cellulase composition,as Cx,Cb,C1 and FPA of JX9 was 82.41、5.71、15.52 and 7.64 U/mL,respectively. |
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| Keywords: | herbal residues cellulose-degrading bacteria screening cellulase |
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