| 分光光度法测定青果多酚的含量 |
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| 引用本文： | 向丽,周铁军,王光西.分光光度法测定青果多酚的含量[J].湖北农业科学,2012(6):1242-1244. |
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| 作者姓名： | 向丽 周铁军 王光西 |
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| 作者单位： | 泸州医学院病原生物学教研室;泸州医学院病理学教研室 |
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| 基金项目： | 泸州市科技计划项目[(2010)128号] |
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| 摘 要： | 以没食子酸为标准品,使用酚试剂,采用分光光度法对青果(Fructus canarii)多酚的含量进行检测。结果表明,酚试剂用量为1.5 mL,与6 mL质量分数10%的Na2CO3溶液混合,30℃反应60 min,于765 nm处测定吸光度,没食子酸浓度在0.5～2.5 mg/L范围内与吸光度有良好的线性关系,回归方程为y=0.121 2x+0.004 0。该方法能较准确地定量分析青果多酚含量,具有简便、快速、稳定,重现性好的优点。
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| 关 键 词： | 分光光度法 酚试剂 青果(Fructus canarii) 多酚 |
Determination of Polyphenol from Fructus canarii by Spectrophotometry |
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| XIANG Lia,ZHOU Tie-junb,WANG Guang-xi.Determination of Polyphenol from Fructus canarii by Spectrophotometry[J].Hubei Agricultural Sciences,2012(6):1242-1244. |
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| Authors: | XIANG Lia ZHOU Tie-junb WANG Guang-xi |
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| Institution: | a(a.Department of Pathogen Biology;b.Department of Pathology,Luzhou Medical College,Luzhou 646000,Sichuan,China) |
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| Abstract: | The polyphenol in Fructus canarii was detected by spectrophotometry with Folin-Ciocalteu reagent and gallic acid as standard.The results showed that the best detection condition was that mixing 1.5 mL Folin-Ciocalteu reagent with 6 mL 10% Na2CO3(m/V),and then reacting for 60 min at 30 ℃ and detected at 765 nm.The linear range of standard curve was 0.5～2.5 mg/L in which the regression equation was y=0.121 2x+0.004 0.The method was simple,fast,stable and reproducible for detecting polyphenol in Fructus canarii. |
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| Keywords: | spectrophotometry Folin-Ciocalteu reagent Fructus canarii polyphenol |
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