| 白桦基因表达半定量RT-PCR中内参基因的选择 |
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| 引用本文： | 戴超,刘雪梅,周菲. 白桦基因表达半定量RT-PCR中内参基因的选择[J]. 经济林研究, 2011, 29(1): 34-39 |
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| 作者姓名： | 戴超 刘雪梅 周菲 |
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| 作者单位： | 东北林业大学生命科学学院,黑龙江,哈尔滨,150040 |
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| 基金项目： | 教育部科学技术研究重点项目，中央高校基本科研业务费专项资金资助 |
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| 摘 要： | 为给调节木质素合成研究的开展提供理论基础,以白桦为研究对象,应用半定量RT-PCR技术分析白桦持家基因18S rRNA、肌动蛋自基因Actin、泛素基因Ubiquitin和微管蛋白基因Tubulin在白桦不同时期及不同部位中的表达.结果表明,18S rRNA,Actin及Ubiquitin基因在各样本间的表达无显著差异...
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| 关 键 词： | 白桦 内参基因 表达分析 |
Selection of internal control genes in semi-quantitative RT-PCR in Betula platyphylla |
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| DAI Chao,LIU Xue-mei,ZHOU Fei. Selection of internal control genes in semi-quantitative RT-PCR in Betula platyphylla[J]. Economic Forest Researches, 2011, 29(1): 34-39 |
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| Authors: | DAI Chao LIU Xue-mei ZHOU Fei |
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| Affiliation: | DAI Chao,LIU Xue-mei,ZHOU Fei(College of Life Science,Northeast Forestry University,Harbin 150040,Heilongjiang,China) |
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| Abstract: | In order to provide theoretical basis for researching lignin synthetic regulation,taking Betula platyphylla as investigated object,expression stability of four housekeeping genes in different tissues and periods were analyzed,which were 18S rRNA,Actin,Ubiquitin and Tubulin,respectively.The results showed that the expression of 18S rRNA,Actin and Ubiquitin were stable,and they could be used as internal control genes to analyze expression level of target genes in B.platyphylla.Tubulin was less stable,and coul... |
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| Keywords: | Betula platyphylla internal control gene expression analysis |
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