| 羊驼酪氨酸酶基因部分cDNA序列的克隆及序列分析 |
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| 引用本文： | 高莉,赵英虎,刘朝亮,董常生.羊驼酪氨酸酶基因部分cDNA序列的克隆及序列分析[J].经济动物学报,2011,15(1):10-13. |
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| 作者姓名： | 高莉 赵英虎 刘朝亮 董常生 |
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| 作者单位： | 1. 中北大学化工与环境学院,太原,030051
2. 山西农业大学,太谷,030801 |
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| 摘 要： | 从3～4龄青年公羊驼背部皮肤中提取总RNA作为模板,采用RT-PCR技术扩增出TYR基因的部分Cdna序列,将其克隆进Pgem-T easy vector中,经限制酶酶切鉴定后进行测序,将测序结果与小鼠、人、猩猩等其它物种的TYR核苷酸序列进行比对,发现本研究获得部分序列为羊驼酪氨酸酶基因第三外显子,该序列比较保守.
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| 关 键 词： | 羊驼 酪氨酸酶 cDNA序列 |
Cloning and Sequencing of Partial cDNA Encoding TYR of Alpaca |
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| GAO Li,ZHAO Ying-hu,LIU Zhao-liang,DONG Chang-sheng.Cloning and Sequencing of Partial cDNA Encoding TYR of Alpaca[J].Journal of Economic Animal,2011,15(1):10-13. |
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| Authors: | GAO Li ZHAO Ying-hu LIU Zhao-liang DONG Chang-sheng |
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| Institution: | GAO Li1,ZHAO Ying-hu1,LIU Zhao-liang1,DONG Chang-sheng2(1.College of Chemical Engineering and Environment,North University of China,Taiyuan 030051,China,2.College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,China) |
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| Abstract: | Total RNA extracted from the dorsal skin of 3-4 years alpaca was used to amplify partial cDNA encoding TYR with RT-PCR technique.The PCR product was cloned into pGEM-T easy vector,and the recombinant plasmid was digested with restriction endonuclease,and then the positive plasmid was sent to be sequenced.After being aligned with the related cDNA sequences of mouse,human,and chimpanzee,it was found the partial cDNA sequences was the third exon of Alpaca TYR and the sequence was conserved. |
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| Keywords: | Lama pacas TYR cDNA sequence |
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