| 副猪嗜血杆菌病原检测及aroA基因PCR-RFLP分型 |
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| 引用本文： | 彭昊,谢宇舟,李军,禤雄标,马春霞,胡帅,杨威,许力干,谢永平,陈泽祥,潘艳. 副猪嗜血杆菌病原检测及aroA基因PCR-RFLP分型[J]. 中国畜牧兽医, 2011, 38(11): 90-94 |
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| 作者姓名： | 彭昊 谢宇舟 李军 禤雄标 马春霞 胡帅 杨威 许力干 谢永平 陈泽祥 潘艳 |
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| 作者单位： | 广西壮族自治区兽医研究所,广西南宁 530001 |
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| 基金项目： | 广西壮族自治区科技厅科技攻关项目(桂科攻0993009-1) |
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| 摘 要： | 本试验应用PCR方法及PCR-RFLP技术对aroA基因在副猪嗜血杆菌病原检测及基因分型中的作用进行探讨。以针对aroA基因的PCR引物成功检测出18株来自广西各地区猪场的副猪嗜血杆菌,敏感性检测结果表明,该PCR方法可检测的最低菌数为102个。对该18株副猪嗜血杆菌进行aroA基因的序列测定,并进行aroA基因的酶切位点分析,筛选出Hind Ⅲ和FokⅠ两种限制性内切酶,利用PCR-RFLP技术对1株血清5型参考菌株与本研究中18株广西菌株aroA基因的完整编码序列进行Hind Ⅲ和FokⅠ限制酶谱分析,结果显示可分为与毒力相关的3种谱型。本研究结果表明,应用PCR方法及PCR-RFLP技术对副猪嗜血杆菌进行检测分析有助于更好地研究副猪嗜血杆菌的生物学特性及aroA基因的功能。
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| 关 键 词： | 副猪嗜血杆菌 病原检测 PCR-RFLP |
Detection and Genotyping of Haemophilus parasuis aroA Gene with PCR-RFLP |
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| PENG Hao,XIE Yu-zhou,LI Jun,XUAN Xiong-biao,MA Chun-xia,HU Shuai,YANG Wei,XU Li-gan,XIE Yong-ping,CHEN Ze-xiang,PAN Yan. Detection and Genotyping of Haemophilus parasuis aroA Gene with PCR-RFLP[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(11): 90-94 |
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| Authors: | PENG Hao XIE Yu-zhou LI Jun XUAN Xiong-biao MA Chun-xia HU Shuai YANG Wei XU Li-gan XIE Yong-ping CHEN Ze-xiang PAN Yan |
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| Affiliation: | Guangxi Veterinary Research Institute, Nanning 530001, China |
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| Abstract: | The PCR assay and PCR-RFLP were applied to study the function of aroA gene on the detection and genotyping of Haemophilus parasuis.18 strains of Haemophilus parasuis isolated from swine in Guangxi were detected successfully by using the PCR assay with primers targeted to aroA gene,and the sensitive test showed that the lowest amount of Hps detectable by this PCR-based test was 102.The aroA gene of the 18 strains was sequenced,and enzyme restriction analysis of the aroA sequences available was performed to d... |
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| Keywords: | Haemophilus parasuis pathogen detection PCR-RFLP |
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