| 叶绿素a测定方法之比较 |
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| 引用本文： | 吴妹英.叶绿素a测定方法之比较[J].福建水产,2011,33(4):61-63. |
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| 作者姓名： | 吴妹英 |
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| 作者单位： | 福建省淡水水产研究所,福建福州,350002 |
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| 基金项目： | 农业部公益性行业(农业)科研专项经费项目(nyhyzx07-043-12); 福建省科技厅公益项目“鳗池藻相人工定向控制技术研究与应用示范”(2009R10012-2) |
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| 摘 要： | 本文比较了3种滤膜（玻璃纤维滤膜、醋酸纤维滤膜、微孔滤膜）、不同提取时间和不同离心温度对叶绿素a含量测定的影响。结果表明，玻璃纤维滤膜叶绿素a测定值显著高于醋酸纤维滤膜和微孔滤膜；叶绿素a最佳提取时间在16h-24h；放人冰块制冷所测得的叶绿素a含量显著高于不放入冰块制冷的含量。
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| 关 键 词： | 叶绿素a 分光光度法 滤膜 提取时间 离心温度 |
Comparisons on detection methods of chlorophyll a |
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| WU Mei-ying.Comparisons on detection methods of chlorophyll a[J].Journal of Fujian Fisheries,2011,33(4):61-63. |
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| Authors: | WU Mei-ying |
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| Institution: | WU Mei-ying(Fujian Provincial Institute of Freshwater Fisheries,Fuzhou 350002,China) |
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| Abstract: | This paper compared the effect of 3 kinds of membrane(glass fiber filtration film,cellulose acetate membrane,microporous membrane),different extraction time and different centrifugal temperatures on chlorophyll a content determination of centrifugal effect.The results showed that the value of glass fiber membrane chlorophyll a determination was significantly higher than that of acetate fiber filter and microporous membrane;the optimal time for extracting chlorophyll a was 14h-24h.The measured chlorophyll a ... |
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| Keywords: | chlorophyll a spectrophotometry membrane extraction centrifugal temperature |
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