| 山羊成纤维生长因子受体（FGFR1）基因的多态性检测 |
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| 引用本文： | 蔡惠芬,陈志,罗卫星,刘若余,张依裕,李敬瑞. 山羊成纤维生长因子受体（FGFR1）基因的多态性检测[J]. 贵州畜牧兽医, 2011, 35(4): 8-11 |
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| 作者姓名： | 蔡惠芬 陈志 罗卫星 刘若余 张依裕 李敬瑞 |
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| 作者单位： | 贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳,550025 |
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| 基金项目： | 贵州省农业科技攻关项目(黔科合NY字[2008]3044) |
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| 摘 要： | 选择与羊同源性较高的牛FGFR1基因组序列（NM_001110207）设计3对特异性引物,采用DNA池PCR直接测序法快速检测黔北麻羊FGFR1基因的多态性,并以内蒙古白绒山羊和关中奶山羊比较,结果表明：在引PCR产物中只有黔北麻羊检测到多态,分别处于外显子5和内含子5的T133C和C211T的碱基突变,而其他2个品种...
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| 关 键 词： | 山羊 FGFR1基因 SNPs |
Polymorphism of Fibroblast Growth Factor Receptor1 (FGFR1) Gene Detection in Goat Breeds |
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| Cai Huifeng,ChenZhi,Luo Weixing,Liu Ruoyu,Zhang Yiyu,Li Jingrui. Polymorphism of Fibroblast Growth Factor Receptor1 (FGFR1) Gene Detection in Goat Breeds[J]. Guizhou Animal Science and Veterinary Medicine, 2011, 35(4): 8-11 |
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| Authors: | Cai Huifeng ChenZhi Luo Weixing Liu Ruoyu Zhang Yiyu Li Jingrui |
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| Affiliation: | Cai Huifeng,ChenZhi,Luo Weixing,Liu Ruoyu,Zhang Yiyu,Li Jingrui(Ministry of Education Key Laboratory for Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,College of Animal Science,Guizhou University,Guiyang Guizhou 550025,China) |
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| Abstract: | Selection cow FGFR1 genome sequence(NM_001110207) which is higher homology with sheep.Three specific primers had been designed.Qianbei ma goat were applied to screen potential single nucleotide polymorphisms(SNPs) in FGFR1 gene based on DNA pooling and direct sequencing of PCR products.It is comparison with neimenggu willi goat and guandong milk goat.The results show that the polymorphism has been detected in the primer FGF-2 PCR product,which mutations at T-133-C of exon 5 and C-211-T of inon 5.But this is... |
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| Keywords: | Goat Fibroblast growth factor receptor 1 Single nucleotide polymorphisms(SNPs) |
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