布氏艾美耳球虫南宁株的分离及鉴定

本试验从南宁市郊区某鸡场感染球虫病的病鸡粪便中收集到混合卵囊,采用改良过的琼脂单卵囊分离法从中分离纯化出2个球虫虫株,并对其中的1个球虫分离株进行了鉴定,鉴定结果为:该株球虫卵囊呈椭圆形或卵圆形,卵囊平均大小为(22.51±0.4790)μm×(15.94±0.5599)μm,平均卵型指数为1.41212±0.06714,裂殖体平均大小为31.33 μm×27.46 μm,潜在期为120 h 50 min,排卵高峰期为感染后第6天,最短孢子化时间为18 h 50 min,寄生部位为小肠中、下段,属中等毒力的球虫虫株。通过将这些测定和观察到的指标与各类文献中所记载的各种鸡艾美耳球虫的特征进行了对比,经过综合分析后,将该分离株球虫鉴定为布氏艾美耳球虫,并且将它命名为布氏艾美耳球虫南宁株,记为Eimeria.brunetti-GXNN。对该虫株的成功分离鉴定,为进一步了解本地球虫虫株的生物学特性,开展鸡球虫病的免疫预防奠定了基础。

Separation and Identification of Eimeria.brunetti-GXNN Strain

A strain of Eimeria coccidia was isolated from suspensions of oocysts by the use of the single oocyst isolation technique. The oocysts of the strain were elliptical in shape and had average dimensions of (22.51±0.4790) μm×(15.94±0.5599)μm. Its prepatent period was 120 h and 50 min, and the peak of oocyst production occurred on the 6^th post infection. Comparing the data of our observation with literature of Eimeria coccidia the isolated strain of Eimeria coccidia was identified as Eimeria. brunetti and was named as Eimeria. brunetti-GXNN. By further researching the characteristics of the postcocious line we would use it as an immunity candidate strain of Eimeria coccidia.