| 口虾蛄肝胰脏超氧化物歧化酶的分离纯化及部分性质的研究 |
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| 引用本文： | 柯佳颖,陈细香,陈寅山,戴聪杰. 口虾蛄肝胰脏超氧化物歧化酶的分离纯化及部分性质的研究[J]. 水产科学, 2011, 30(9) |
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| 作者姓名： | 柯佳颖 陈细香 陈寅山 戴聪杰 |
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| 作者单位： | 1. 泉州师范学院化学与生命科学学院,福建泉州,362000
2. 福建师范大学生命科学学院,福建福州,350007 |
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| 基金项目： | 福建省高校服务海西建设重点项目(No.A101); 福建省教育厅A类科技项目(JA09213); 泉州市科技计划项目(2010N28) |
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| 摘 要： | 经热变性,硫酸铵二步分级沉淀,再经Sephadex G-100分子筛和DEAE-Sepharose离子交换层析,从口虾蛄肝胰脏中分离纯化得到超氧化物歧化酶。试验获得的酶紫外吸收峰在268 nm处,比活力为983.8 U/mg,提纯倍数为378.4。该酶分子量约31.5 ku,是由2个分子量均为15.6 ku的亚基组成的二聚体。该酶在30～60℃稳定性较好,最适pH值为6.7。Li+、K+、Ca2+、Zn2+对该酶活性的影响总趋势是抑制作用。紫外灯照射使酶活性呈现递减的变化趋势。
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| 关 键 词： | 口虾蛄 肝胰脏 超氧化物歧化酶 纯化 性质 |
Purification and Characterization of Superoxide Dismutase in Hepatopancreas from Mantis Shrimp Oratosquilla oratoria |
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| KE Jia-ying,CHEN Xi-xiang,CHEN Yin-shan,DAI Cong-jie. Purification and Characterization of Superoxide Dismutase in Hepatopancreas from Mantis Shrimp Oratosquilla oratoria[J]. Fisheries Science, 2011, 30(9) |
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| Authors: | KE Jia-ying CHEN Xi-xiang CHEN Yin-shan DAI Cong-jie |
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| Affiliation: | KE Jia-ying1,CHEN Xi-xiang1,CHEN Yin-shan 2,DAI Cong-jie11.Bioengineering Department,Quanzhou Normal University,Quanzhou 362000,China,2.College of Life Sciences,Fujian Normal University,Fuzhou 350007,China) |
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| Abstract: | The superoxide dismutase(SOD)was purified in hepatopancreas from mantis shrimp Oratosquilla oratoria by extraction,heating denaturation,fraction with saturated ammonium sulfate precipitation,and gel chromatography on the Sephadex G-100 column and followed by ion exchange chromatography on the DEAE-Sepharose colum.The results showed that the SOD had the ultraviolet absorption peak at 268 nm.The specific activity of the enzyme was found to be 983.8 U/mg,with purification factor of 378.4,and molecular weight o... |
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| Keywords: | Oratosquilla oratoria hepatopancreas superoxide dismutase purification characterization |
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