Development of species‐specific PCR primers on rDNA for the identification of European Armillaria species |
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| Authors: | G Sicoli J Fatehi J Stenlid |
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| Institution: | 1. Dipartimento di Biologia e Patologia vegetale, Università degli Studi di Bari, via G. Amendola, 165/A, I‐70 126 Bari, Italy. E‐mail: g.sicoli@agr.uniba.it;2. Department of Molecular Evolution, Uppsala University, Norbyv?gen 18C, 752 36 Uppsala, Sweden;3. Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, PO Box 7026, SE‐750 07 Uppsala, Sweden |
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| Abstract: | Attempts to design species‐specific PCR primers from six European Armillaria species in the ribosomal RNA genes are reported. Primers were developed on the basis of the nucleotide sequence variability of the internal transcribed spacers (ITS) and the intergenic spacer (IGS1) of the ribosomal DNA. Four sets of primers gave specific PCR products for Armillaria tabescens, Armillaria mellea and Armillaria ostoyae. However, due to the high sequence similarities between Armillaria borealis and Armillaria ostoyae and between Armillaria cepistipes and Armillaria gallica no species specific amplification was obtained for these taxa. |
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