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| 致病疫霉(Phytophthora infestans)的分离与培养方法 | |
| 引用本文： | 毕朝位,黎艳平,罗国全. 致病疫霉(Phytophthora infestans)的分离与培养方法[J]. 中国农学通报, 2005, 21(10): 306-306 |
| 作者姓名： | 毕朝位 黎艳平 罗国全 |
| 作者单位： | 1. 西南农业大学植保学院,重庆,400716 2. 重庆永川市农技中心,402160 3. 重庆壁山县植保站,402760 |
| 基金项目： | 重庆市自然科学基金资助项目，西南农业大学青年基金资助项目(编号:2004专03) |
| 摘 要： | 针对不同地区的病样，分别采用直接分离法、保湿培养分离法、薯片夹叶分离法、薯片诱导分离法等分离方法，利用选择性培养基SRA进行分离，通过挑取菌落边缘菌丝尖端于斜面培养基进行纯化，共获得重庆马铃薯和番茄主产区186个致病疫霉菌株。测定代表性菌株在不同培养基上的菌落生长和产孢量。结果表明，致病疫霉在RSA上生长最快，RA和RSB次之；在RSB上产孢最多，RTA次之。 |
| 关 键 词： | 致病疫霉 分离方法 培养基 |
| 收稿时间： | 2005-06-12 |
| 修稿时间： | 2005-06-122005-06-15 |
The Study on Isolating and Culturing Phytophthora infestans |
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| Bi Chaowei,Li Yanping,Luo Guoquan. The Study on Isolating and Culturing Phytophthora infestans[J]. Chinese Agricultural Science Bulletin, 2005, 21(10): 306-306 | |
| Authors: | Bi Chaowei Li Yanping Luo Guoquan |
| Affiliation: | 1. College of plant protection, Southwest Agri. Univ. Chongqing 400716; 2. The Center of Agri. Tech. of Yongchuan.Chongqing 402160; 3. plant Protection Station of Bishan. Chongqing 402760 |
| Abstract: | Routine isolation way, dew chamber way, disease leaf claping with potato slices and induction with potato tuber were tested, besed on semi-composed medium of RSA plus several antibiotics. Total 186 isolates from representive areas of potato and tomato in Chongqing were obtained and purified through cutting the top of mycelium of pathogen. Bioassay of typical isolate was carried out to determine the growth and sporulation of Phytophthora infestans. The results showed that the growth rate of Phytophthora infestans on tested media is as following order: RSA>RA>RSB>SVA>RTA>LVA>VA>MA>PSA>PDA; RSB is best for sporulation, then RTA. |
| Keywords: | Phytophthora infestans Isolating and Culturing Selective medium |
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