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| 靶向IRES序列的siRNA对口蹄疫病毒体外增殖抑制效果 | |
| 引用本文： | 崔丽瑾,王兴龙,任晓慧,任林柱,郎需龙,杨艳玲,李晓艳,卜朝阳.靶向IRES序列的siRNA对口蹄疫病毒体外增殖抑制效果[J].中国兽医学报,2010,30(5). |
| 作者姓名： | 崔丽瑾 王兴龙 任晓慧 任林柱 郎需龙 杨艳玲 李晓艳 卜朝阳 |
| 作者单位： | 1. 军事医学科学院军事兽医研究所,吉林长春130062;中国疾病预防控制中心病毒病所,北京100052 2. 军事医学科学院军事兽医研究所,吉林长春,130062 3. 河北农业大学海洋学院,河北秦皇岛,066003 4. 吉林大学畜牧兽医学院,吉林长春,130062 |
| 基金项目： | 吉林省科技发展资助项目(20050549) |
| 摘 要： | 为探讨靶向核糖体进入位点(IRES)区域的siRNA在真核细胞中对FMDV复制的抑制作用,对FMDV WFL株的IRES序列进行了保守性分析和二级结构预测,选择了靶向IRES保守的功能核心区的病毒复制原点附近的2个干扰靶位,设计并构建了shRNA真核表达质粒,转染到IBRS-2细胞后感染FMDV,通过双抗夹心ELISA和real-timeRT-PCR检测FMDV复制情况,结果表明,针对IRES区域的RNAi能够在一定程度上抑制FMDV的复制。 |
| 关 键 词： | 口蹄疫病毒 RNA干扰 IRES序列 IBRS-2细胞 抑制 |
Inhibition of food-and-mouth disease virus by siRNAs targeting IRES sequence in vitro |
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| CUI Li-jin,WANG Xing-long,REN Xiao-hui,REN Lin-zhu,LANG Xu-long,YANG Yan-ling,LI Xiao-yan,BU Zhao-yang.Inhibition of food-and-mouth disease virus by siRNAs targeting IRES sequence in vitro[J].Chinese Journal of Veterinary Science,2010,30(5). | |
| Authors: | CUI Li-jin WANG Xing-long REN Xiao-hui REN Lin-zhu LANG Xu-long YANG Yan-ling LI Xiao-yan BU Zhao-yang |
| Institution: | CUI Li-jin1,2,WANG Xing-long1,REN Xiao-hui3,REN Lin-zhu4,LANG Xu-long1,YANG Yan-ling1,LI Xiao-yan1,BU Zhao-yang1 (1.The Military Veterinary Institute,Academy of Military Medical Sciences of PLA,Changchun 130062,China,2.National Institute for Viral Disease Control , Prevention,China CDC,Beijing 100052,3.College of Ocean,Hebei Agricultural University,Qinhuangdao,Hebei 066003,4.College of Animal Science , Veterinary Medicine,Jilin University,China) |
| Abstract: | To investigate interference of FMDV replication in eukaryocyte cells by small interfering RNAs (siRNAs) targeting internal ribozyme entry site(IRES),conservative analysis and RNA secondary structure prediction of FMDV IRES were accomplished and two target sequences of siRNAs which were highly conservative and located in the loop region of IRES RNA secondary structure were chosen.Two siRNA expressing plasmids were constructed and transfected to IBRS-2 cells followed by FMDV infected.The results of CPE,ELISA ... |
| Keywords: | foot-and-mouth disease virus RNA interference IRES sequence IBRS-2 cell inhibition |
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