攀西黑山羊群体的AFLP遗传多样性研究

 研究采用随机片段长度多态性分析了攀西地区的5个黑山羊品种（类群）的遗传多样性。结果表明：15对引物共扩增出1003条多态条带，多态频率平均为99.01%，5个黑山羊群体的遗传多样性指数变异范围为0.0136~0.2131，其中建昌黑山羊平均遗传多样性指数最高（0.1346），攀枝花黑山羊次之（0.1329），乐至黑山羊最低（0.1101）。5个群体遗传距离范围为0.0062~0.0281，攀枝花黑山羊与建昌黑山羊之间的遗传距离最小（DR=0.0062）,云岭黑山羊与攀枝花黑山羊之间的遗传距离次之（DR=0.0128），乐至黑山羊与金堂黑山羊间遗传距离最大（DR=0.0281），金堂黑山羊与其它4个类群遗传距离较大（DR=0.0173-0.0281），说明攀枝花黑山羊与建昌黑山羊的亲缘关系最近，与云岭黑山羊的亲缘关系较近，乐至黑山羊与金堂黑山羊的亲缘关系最远，金堂黑山羊与其它4个类群遗传距离均比较远。聚类结果与群体地理分布、生态条件差异情况基本一致。

Study on Genetic Diversity Based on AFLP Markers on Black Goat in the West of Panzhihua

In this study, AFLP(Amplified Fragment Length Polymorphism) was employed to analyze genetic diversity of 5 black goat populations in Panzhihua. 15 primer combinations were used to amplify 1003 polymorphic markers, the average polymorphic frequency is 99.01%. The genetic diversity indexes of the 5 black goat populations are from 0.0136 to 0.2131, the maximum and minimum average genetic diversity indexes are Jianchang（0.1346）and Lezhi（0.1101） respectively. The genetic distance is from 0.0062 to 0.0281. Aa a result, Jianchang and Panzhihua  black goats are clustered into a class, Yunling and Lezhi black goats are clustered into a class, the above two classes are clustered into a large one; the large class is clustered with Jingtang black goat into one class. The of AFLP could reflect the influence of environment conditions on genetic diversity.