Isolation,selection and evaluation of Rhizobium under controlled conditions

Abstract

Rhizobia were screened in growth chamber and greenhouse tests with the host plants: bean (Phaseolus vulgaris L.), lentil (Lens esculenta Moench.), cowpea (Vigna unguiculata (L.) Walp) and peanut (Arachis hypogaea L.). Rhizobial isolates varied in effectiveness, time to nodulation, and host plant specifities. Initial screening procedures in plastic growth pouches allowed selection of infective strains of Rhizobium. These tests enabled the selection of a small number of highly effective isolates for more critical evaluation. Highly significant correlations (p=0.01) were obtained between shoot dry weight and total nitrogen, suggesting that the technique was applying sufficient selection pressure to differentiate variation in N₂ fixation among strains of Rhizobium. Results indicate that highly effective rhizobia can be efficiently selected under controlled conditions following a step‐wise procedure.