Methods to Estimate the Protection of Soil Organic Carbon within Macroaggregates, 1: Does Soil Water Status Affect the Estimated Amount of Soil Organic Carbon Protected inside Macroaggregates?

The additional mineralized soil organic carbon (SOC) after soil crushing is considered to be the amount of SOC protected within aggregates (>200 μm). This study investigated the effect of soil moisture in crushed and uncrushed soil samples on the calculated amounts of protected SOC in five tropical soils (Arenosol, two Ferralsols, Nitisol, and Vertisol). No differences in soil moisture optimum were observed between crushed and uncrushed soil samples, except in clayey soils with high SOC contents and high SOC mineralization rates (Nitisol and Vertisol). Crushing the soil increased soil respiration by 0.9 to 2.4 times. Soil moisture seemed to be a confounding factor in estimation of the SOC-protected amount only in soil with a high amount of protected SOC or with a low macroaggregate stability (Ferralsol and Vertisol). In these soils, the amount of protected SOC could be influenced by the method used to estimate it.