In vitro Fertilization of Bovine Oocytes Exposed to Bovine Herpesvirus 1 (BHV-1) |
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Authors: | A Bielanski C Dubuc |
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Institution: | Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario, Canada |
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Abstract: | Contents: Cumulus-oocyte complexes were matured for 24 hours in the presence or absence of bovine herpes virus-1 (BHV-1, 106 TCID50/ml), fertilized in vitro and then co-cultured on oviductal cells to the blastocyst stage. The percentages of oocytes which cleaved were 48% (n = 905) for oocytes matured in the presence of BHV-1, and 51% (n = 1004) for the control oocytes. Comparing BHV-1-treated to control, the percentages of cleaved oocytes which developed to the blastocyst stage were 29% and 31%, respectively (p > 0.05). Embryos produced from the experimental group of oocytes tested positive for BHV-1. |
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Keywords: | embryo in vitro fertilization bovine herpesvirus-1 |
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