芦荟试管微克隆繁育中芽增殖体系的建立

研究了库拉索芦荟茎尖组织、叶盘组织和茎盘组织的离体培养特性,发现芦荟茎尖组织芽增殖效率较低,叶盘组织无再生能力,而茎盘组织是芦荟试管克隆芽增殖系建立的最佳外植体。利用茎盘组织建立微克隆增殖系与茎盘组织在茎段上的位置有关,库拉索短宿茎的中部茎段不定芽形成能力最强。细胞分裂素是诱导库拉索芦荟芽增殖的主要激素,但质量浓度过高,不定芽生长弱,玻璃化现象严重。1.0mg/L6-BA,1.5mg/LKT和0.5mg/LNAA组合的MB培养基(该培养基由MS培养基大量元素、微量元素、铁盐,B5培养基的有机物质构成)是库拉索芦荟试管苗增殖的最佳培养基。

Organogenesis and plantlet regeneration in vitro of CURACAO aloe micropropagation

The research of cultural character in vitro of CURACAO Aloe was carried out using young stem apex,leaf segments and stem segments as initial explants.The result indicated that young stem apex had lower reproducing rate while leaf segments had no ability to reproduce.Stem segment was the best explant for the plantlet regeneration in vitro of CURACAO Aloe.But the multiplication rate had relationship with the site where the stem segments set.The segment in the middle of the stem had more potent to produce adventitious bud.Cytokinin was the main exogenous hormone which induced the plantlet regeneration.But high concentration resulted in adventitious bud poor growth and severe vitrification.The best medium for the plantlet regeneration of CURACAO Aloe was MB medium (MB medium contains the macroelement,microelement,molysite of MS medium and organic matter of B₅ medium ) with 6-BA 1 mg/L,KT 1.5 mg/L,NAA 0.5 mg/L.