Four-layer enzyme immunoassay (EIA) detection of differences in IgG, IgM and IgA antibody response to Aujeszky's disease virus in infected and vaccinated pigs |
| |
| Authors: | L Rodák B Smíd L Valícek E Jurák |
| |
| Affiliation: | 1. Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, 08193 Cerdanyola Del Vallès, Spain;2. IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Cerdanyola Del Vallès, Spain;1. Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a la Victoria km 0.6 C.P. 83304, Hermosillo, Sonora, Mexico;2. Departamento de Ciencias Químico Biológicas, Universidad de Sonora, 83000, Hermosillo, Sonora, Mexico;1. Department of Public Health Sciences, University of Virginia, PO Box 801379, Carter Harrison Research Building MR-6, 345 Crispell Drive, Room 2520, Charlottesville, VA 22908-1379, USA;2. Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, PO Box 801379, Carter Harrison Research Building MR-6, 345 Crispell Drive, Room 2520, Charlottesville, VA 22908-1379, USA;1. Graduate Program of Animal Sciences – Universidade do Estado de Santa Catarina (UDESC), Chapecó, Santa Catarina, Brazil;2. Department of Animal Sciences - Universidade do Estado de Santa Catarina (UDESC), Chapecó, Santa Catarina, Brazil;3. Department of Microbiology and Parasiotology, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil;4. EMBRAPA Swine and Poultry, Concórdia, SC, Brazil;5. Laboratório de Micologia da Universidade de São Paulo, Brazil;1. Istituto di Sostenibilità Applicata All’ambiente Costruito, Scuola Universitaria Professionale della Svizzera Italiana, Campus Trevano, Via Trevano, CH-6952 Canobbio, Switzerland;2. Architecture, Built Environment and Construction Engineering Department, Politecnico di Milano, Via Bonardi 9, 20133 Milano, Italy |
| |
| Abstract: | The use of the four-layer enzyme immunoassay (EIA) for the detection of IgG, IgM and IgA antibodies against Aujeszky's disease virus in blood and oropharyngeal swabs of infected and vaccinated pigs is described. Mean antibody titres obtained using the four-layer EIA were 6.1 and 3829 times higher compared with the indirect enzyme-linked immunosorbent assay (ELISA) and virus neutralization (VN) test, respectively. The VN test detected mainly IgG antibodies, while the IgM antibodies did not react. Using the EIA, the first antiviral antibodies in sera were demonstrated on Days 5-7 after infection or vaccination. Up to the 7th day, demonstrable antibodies were almost exclusively of the IgM class. In infected pigs high titres of IgM antibodies were still detected on Day 18, while in vaccinated animals they were absent by this time. Antibodies of the IgG class appeared in infected pigs sooner (Day 7) than in vaccinated pigs (Day 10) and reached higher mean titres. Antibodies of the IgA class were demonstrable from Day 10 only in samples from infected pigs. Similar antibody dynamics and distribution were detected in oropharyngeal swabs, except that the IgG and IgM titres were roughly 100 times lower than in sera. However, titres of IgA antibodies in oropharyngeal swabs were two times higher than in sera. The greatest differences between both groups of animals were recorded on Day 18; in the infected pigs, IgG, IgM and IgA antibodies were present in sera and oropharyngeal swabs at that time, while in vaccinated pigs only IgG antibodies were demonstrable. The effect of infection and vaccination on the pattern of the immune response as well as the importance of the detection of individual immunoglobulin classes for the specificity of the enzyme immunoassay are discussed. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
