| 转雪花莲凝集素酶基因枸杞对蚜虫的抑制作用 |
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| 引用本文： | 罗青,巫鹏举,李晓莺,贝盏临,曲玲,曹有龙.转雪花莲凝集素酶基因枸杞对蚜虫的抑制作用[J].植物保护,2007,33(1):65-67. |
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| 作者姓名： | 罗青 巫鹏举 李晓莺 贝盏临 曲玲 曹有龙 |
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| 作者单位： | 宁夏枸杞发展工程技术研究中心 银川750002 |
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| 基金项目： | 国家科技攻关项目;宁夏自然科学基金 |
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| 摘 要： | 利用农杆菌介导法将雪花莲外源凝集素酶基因导入枸杞叶片,获得了完整的转基因植株。对36个经PCR检测为阳性的转基因株系进行了两年抗蚜虫筛选试验,结果表明:蚜口密度抑制率在13.19%～95.4%,平均蚜口密度抑制率为78.59%。
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| 关 键 词： | 枸杞 雪花莲外源凝集素酶基因 转化 蚜虫 抑制 |
| 修稿时间： | 2006-03-172006-05-30 |
Inhibition effects of the transgenic Lycium plants expressing snowdrop lectin on development of aphid population |
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| Luo Qin;Wu Pengju;Li Xiaoying;Bei Zhanlin;Qu Ling;Cao Youlong.Inhibition effects of the transgenic Lycium plants expressing snowdrop lectin on development of aphid population[J].Plant Protection,2007,33(1):65-67. |
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| Authors: | Luo Qin;Wu Pengju;Li Xiaoying;Bei Zhanlin;Qu Ling;Cao Youlong |
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| Institution: | Ningxia Wolfberry Developmental Engineering and Technology Research Center, Yinchuan 750002, China |
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| Abstract: | Agrobacterium-mediated transformation of Lycium was studied to prevent the pest aphid.The gene gna was successfully transferred into Lycium by using leaf dish,and a large number of completely transgenic plants were obtained.Thirty-six transgenic Lycium plants that had been confirmed as being gna-integrated by PCR detection were tested for resistance to aphids in two years.The results showed that the inhibition rate of aphid population was 13.19%-95.4%,78.59% on average. |
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| Keywords: | Lycium barbarum L gna gene transformation aphid inhibition |
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