2种单组份双生病毒与异源卫星DNA的互作

 烟草曲茎病毒(Tobacco curly shoot virus,TbCSV) Y35分离物(Y35)和中国番茄黄化曲叶病毒(Tomato yellow leaf curl China virus,TYLCCNV) Y10分离物(Y10)是从云南烟草上分离的2种单组份双生病毒,分别伴随卫星DNAβ(Y35β及Y10β)分子。将Y35和Y10分别伴随其同源或异源卫星(Y35β或Y10β)接种本氏烟和心叶烟,症状观察表明,无论Y10还是Y35伴随异源卫星DNAβ时引起的症状均与其伴随同源DNAβ引起的症状明显不同,且Y10β伴随Y35致病性最强。Southern印迹结果表明,Y10β及Y35β均可被异源辅助病毒稳定复制,但其复制水平明显低于伴随同源病毒时的水平,且植株中病毒和卫星的积累量并不完全与症状严重程度直接相关。

Interactions between two monopartite begomoviruses and noncognate satellite DNA

Tobacco curly shoot virus (TbCSV) Y35 isolate and Tomato yellow leaf curl China virus (TYLCCNV) Y10 isolate isolated from tobacco in Yunnan Province, were monopartite begomoviruses and associa-ted with satellite DNAβ molecules Y35β and Y10β respectively. Nicotiana benthamiana and N. glutinosa were inoculated with the combinations of Y35 or Y10 associated with cognate or noncognate satellite DNAβ (Y35β or Y10β) respectively. Symptoms induced by Y10 or Y35 with their cognate DNAβ were different from those induced by co-infection with noncognate DNAβ, and high pathogenicity was found in plants inoculated with Y10β and Y35. Southern blot results showed that both Y10β and Y35β could be stablly trans-replicated by noncognate geminivirus, but DNAβ molecules associated with noncognate helper virus accumulated in plants at a lower level compared with those associated with cognate helper virus. No direct correlation existed between symptom severity and accumulation level of virus and satellite in plants.