The onset and course of estrus in young heifers]

In 1990 three experiments were performed in heifers 184 to 505 day-old with the weight 190 to 390 kg to test some biotechnical methods while studying the level of reproduction functions. Heifers were examined in 4 to 24-hour intervals by direct observations of animal behaviour, by clinical and gynaecological examinations of larger animals. The progesterone concentration in the peripheral blood was determined by the RIA method and simultaneously with the blood samplings, the values of impedance of vaginal mucosa were measured by means of several types of the Estral device (CSFR) for cattle. To detect the functional responses to prostaglandins administered, the following forms of substances were used: racemic mixture of optically active forms D (+) and L (-) in the Czechoslovak preparation Oestrophan inj. Spofa (0.25 mg of cloprostenol in 1 ml of solution) and the dextrorotatory D isomer--cloprostenol (0.075 mg in 1 ml of solution) in the Czechoslovak preparation Remophan inj. Spofa. Evaluating the results of the group 1 based on clinical and ethological examinations, the values of impedance of vaginal mucosa and progesterone levels (Tab. I), the functional maturity of sexual organs in heifers were determined. No significant differences were found while compared the luteolytic effects between D-cloprostenol in the Remophan preparation and the conventional mixture of optically active forms of Oestrophan. All methods used confirm the functional maturity of sexual organs (including a response to prostaglandin analogues administered) in heifers 11-12 month old. The values of impedance of vaginal mucosa varied during oestrus in some heifers and this fact is to be taken into account in practice.(ABSTRACT TRUNCATED AT 250 WORDS)