| 一种高质量枇杷基因组DNA提取方法 |
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| 引用本文： | 杨芩,付燕,邓群仙,王永清,陶炼,刘露,李性苑,张文华.一种高质量枇杷基因组DNA提取方法[J].北方园艺,2010(20):126-129. |
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| 作者姓名： | 杨芩 付燕 邓群仙 王永清 陶炼 刘露 李性苑 张文华 |
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| 作者单位： | 凯里学院,环境与生命科学学院,贵州,凯里,556011;黔东南民族职业技术学院,贵州,凯里,556000;四川农业大学,园艺学院,四川,雅安,625014;凯里学院环境与生命科学学院,贵州,凯里,556011 |
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| 基金项目： | 贵州省教育厅自然科学基金 |
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| 摘 要： | 针对枇杷叶片富含多糖、多酚及其它次生代谢物质的特点,采用改良CTAB法,通过对抽提液、沉淀液和解离缓冲液进行逐步筛选,对其基因组总DNA进行提取,并对DNA进行电泳检测、含量测定和ISSR分析。结果表明:该方法从‘大五星’枇杷幼叶、成熟叶片、花药、组培苗,与幼果中的胚珠,以及栎叶枇杷的幼叶与成熟叶片7种材料提取的基因组总DNA纯度和完整性都较好,OD260/OD280值均在1.8~2.0之间,无降解现象,RNA去除干净,能被限制性内切酶完全消化;其ISSR分析条带清晰,多态性好,表明此方法提取的枇杷基因组总DNA质量较高。
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| 关 键 词： | 枇杷 基因组DNA ISSR |
A Method for Excellent Quality DNA Extraction of Loquat |
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| YANG Qin,FU Yan,DENG Qun-xian,WANG Yong-qing,TAO Lian,LIU Lu,LI Xing-yuan,ZHANG Wen-hua.A Method for Excellent Quality DNA Extraction of Loquat[J].Northern Horticulture,2010(20):126-129. |
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| Authors: | YANG Qin FU Yan DENG Qun-xian WANG Yong-qing TAO Lian LIU Lu LI Xing-yuan ZHANG Wen-hua |
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| Institution: | YANG Qin1,FU Yan2,DENG Qun-xian3,WANG Yong-qing3,TAO Lian3,LIU Lu3,LI Xing-yuan1,ZHANG Wen-hua(1.College of Environmental and Life Science,Kaili University,Kaili,Guizhou 556000,2.Qiandongnan Nationality Professional Technology College,3.College of Horticulture,Sichuan Agricultural University,Ya'an,Sichuan 625014) |
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| Abstract: | The leaves of loquat are rich in polyhexose,polyphenols and other secondary metabolism compounds which prevent DNA extraction.The genomic DNA samples of loquat were isolated by improved CTAB method which based on optimizing extraction,deposition and dissociation.The purity and quantity of DNA was evaluated by agarose gel electrophoresis,UV scanning and ISSR analysis.The results showed that the genomic DNA extracted from Yong leaves and mature leaves of Dawuxing and Oakleaf loquat,and anther,seedlings of tis... |
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| Keywords: | Loquat genomic DNA ISSR |
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