|
|||||
|
|
| Agrobacterium tumefaciens-mediated GUS gene transfer to Sophora japonica L. | |
| 作者姓名： | Zhang Xiao-ying Wang Hua-fang Yin Wei-lun Zhu Zhen College of Biological Sciences and Biotechnology Beijing Forestry University Beijing P. R. China Beijing Langshan Nursery Beijing P. R. China Institute of Genetics Chinese Academy of Sciences Beijing P. R. China |
| 作者单位： | Zhang Xiao-ying Wang Hua-fang Yin Wei-lun Zhu Zhen College of Biological Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，P. R. China Beijing Langshan Nursery，Beijing 100042，P. R. China Institute of Genetics，Chinese Academy of Sciences，Beijing 100101，P. R. China |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | Agrobacterium-mediated genetic transformation of Sophora japonica was standardized using the Agrobacterium tumefaciens strain LBA4404 that harbored the binary vector pBI121 containing genes forβ-glucuronidase (GUS) and neomycin phos-photransterase (nptⅡ). S. japonica transformants were selected by the ability of the leaf explants to produce kanamycin-resistant calli that regenerated into kanamycin-resistant plantlets. Successful transformation was confirmed by histochemical assay for GUS activity, PCR analysis and Southern blot. The period of nearly two months was required for the regeneration of transgenic plantlets from the explants. The transformed plants resembled their parents in morphology. |
| 关 键 词： | β-glucuronidase (GUS) Sophora japonica Agrobacterium tumefaciens neomycin phosphotransterase (nptⅡ) transformation |
| 收稿时间： | 2006-6-6 |
Agrobacterium tumefaciens-mediated GUS gene transfer to Sophora japonica L. |
|
| Zhang Xiao-ying Wang Hua-fang Yin Wei-lun Zhu Zhen College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing ,P. R. China Beijing Langshan Nursery,Beijing ,P. R. China Institute of Genetics,Chinese Academy of Sciences,Beijing ,P. R. China.Agrobacterium tumefaciens-mediated GUS gene transfer to Sophora japonica L.[J].Forestry Studies in China,2006,8(4):6-9. | |
| Authors: | Zhang Xiao-ying Wang Hua-fang Yin Wei-lun Zhu Zhen |
| Institution: | 1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China;Beijing Langshan Nursery, Beijing 100042, P. R. China 2. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China 3. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, P. R. China |
| Abstract: | Agrobacterium-mediated genetic transformation of Sophorajaponica was standardized using the Agrobacterium tumefaciens strain LBA4404 that harbored the binary vector pBI121 containing genes for β-glucuronidase (GUS) and neomycin phosphotransterase (npt Ⅱ). S. japonica transformants were selected by the ability of the leaf explants to produce kanamycin-resistant calli that regenerated into kanamycin-resistant plantlets. Successful transformation was confirmed by histochemical assay for GUS activity, PCR analysis and Southern blot. The period of nearly two months was required for the regeneration of transgenic plantlets from the explants. The transformed plants resembled their parents in morphology. |
| Keywords: | Sophora japonica Agrobacterium tumefaciens transformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国林学》浏览原始摘要信息 | |
| 点击此处可从《中国林学》下载免费的PDF全文 | |