毛竹根系中5-脱氧独角金醇的超高效液相色谱分析

以毛竹Phyllostachys edulis根为试验材料，首次建立了竹子根系内源激素独角金内酯的提取和测定方法。毛竹根经研磨、提取、分离、纯化等获得供试样品，采用液相色谱仪-PDA检测器定性和定量分析了5-脱氧独角金醇（5-deoxystrgol，5-DS）。结果表明:应用该方法，样品制备简便，分离效果好，方法的线性范围及精密度、准确度和回收率都能满足毛竹根系中痕量成分定性和定量分析的要求。根据5-DS的保留时间以及光谱图对样品中色谱峰定性，确定毛竹根系中含有5-DS；根据5-DS的标准曲线采用外标法对毛竹根系提取物中的5-DS进行定量测定，毛竹根系中5-DS质量分数为（6.62 0. 27 ） gkg－1。图2表2 参11

Determination of strigolactones extracted from root of Phyllostachys edulis by ultra performance liquid chromatography

A simple and highly sensitive method was first developed for the determination of strigolactones extracted from roots of Phyllostachys edulis by ultra performance liquid chromatography （UPLC） with ultraviolet （UV） detection；5-deoxystrgol（5-DS） one kind of strigolactones was conducted qualitative and quantative analysis. This method presented good recovery （80.5%－95.8%），precision （RSD＜12%），and linear relations 0.5-40.0 mgL－1）. The extract from roots of Phyllostachys edulis was determined as 5-deoxystrgo according to the retention time and spectrum of analyses. The content of 5-deoxystrgo in roots of Phyllostachys edulis is （6.62 0.27） gkg－1. ［Ch，2 fig. 2 tab. 11 ref.］