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雄性食蟹猴生殖系统中Ghrelin的分布定位
引用本文:李鑫,宋尚桥,马围围,郑福,曾素先,孙翠翠,严瑾,黎宗强. 雄性食蟹猴生殖系统中Ghrelin的分布定位[J]. 野生动物学报, 2020, 0(2): 325-330
作者姓名:李鑫  宋尚桥  马围围  郑福  曾素先  孙翠翠  严瑾  黎宗强
作者单位:广西大学动物科学技术学院;南宁市江南区沙井街道办事处水产畜牧兽医站
基金项目:广西壮族自治区科技计划项目-重点研发计划(桂科AB16380072)。
摘    要:本文采用免疫组织化学染色方法,探讨ghrelin在雄性食蟹猴生殖系统内的分布定位。通过对ghrelin免疫阳性细胞在生殖系统中分布部位、含量及细胞形态等方面的研究,为今后ghrelin在食蟹猴体内的功能研究奠定形态学基础。免疫组化染色发现,ghrelin阳性细胞在食蟹猴的生殖系统中有分布。Ghrelin免疫阳性细胞被染为棕色到棕黑色,主要分布于睾丸、附睾及输精管中,精囊腺中无ghrelin阳性细胞分布。Ghrelin阳性细胞在组织中多呈散在分布。细胞大小不一、形态各异,多呈圆形、卵圆形、锥体形、长柱形及其他不规则形。

关 键 词:GHRELIN  食蟹猴  生殖系统  免疫组化定位

Study on Immunohistochemical Localization of Ghrelin in the Reproductive System of Male Macaca fascicularis
LI Xin,SONG Shangqiao,MA Weiwei,ZHENG Fu,ZENG Suxian,SUN Cuicui,YAN Jin,LI Zongqiang. Study on Immunohistochemical Localization of Ghrelin in the Reproductive System of Male Macaca fascicularis[J]. Chinese Journal of Wildlife, 2020, 0(2): 325-330
Authors:LI Xin  SONG Shangqiao  MA Weiwei  ZHENG Fu  ZENG Suxian  SUN Cuicui  YAN Jin  LI Zongqiang
Affiliation:(College of Animal Science and Technology,Guangxi University,Nanning,530005,China;Shajing Subdistrict Office,Jiangnan District,Nanning City Aquatic Animal Husbandry and Veterinary Station,Nanning,530033,China)
Abstract:We used immunohistochemistry to investigate the localization of ghrelin in the reproductive system of Macaca fascicularis.Immunohistochemical staining showed that ghrelin-positive cells were recorded in the reproductive system of M.fascicularis.Here,ghrelin-immunoreactive cells were stained brown to brown-black,and were mainly distributed in the testis,epididymis and spermaduct,but not in the seminal vesicle.Ghrelin cell distribution was scattered in tissues.The sizes and shapes of cells varied but mainly were round,oval,spindle-shaped,columnar,and of other irregular shapes.
Keywords:Ghrelin  Macaca fuscicularis  Reproductive system  Immunohistochemical localization
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