芥菜型油菜叶片黄化突变体的初步研究

为了揭示芥菜型油菜叶片黄化突变体的黄化机理,通过田间统计、叶绿素测定和同工酶分析,初步研究了芥菜型油菜叶片黄化突变体L638-y的生物学特性。结果表明,与原始材料L638-g相比,黄化突变体L638-y的子叶宽度、株高、一次分枝数、单株产量均减少,始花期推迟1 d,生育期减少1 d,说明叶片黄化突变对植株发育的进度影响较大;黄化突变体L638-y的叶绿素a、叶绿素b和类胡萝卜素含量均明显降低,特别是叶绿素b含量锐减,说明突变影响了叶绿素a向叶绿素b的转变;黄化突变体L638-y与原始材料L638-g叶片的过氧化物酶和酯酶同工酶的条带数目以及条带亮度均无明显差异。提示芥菜型油菜黄化突变体L638-y可能为缺总Chl型,导致叶片缺绿的原因为叶绿素代谢异常。

Primary investigation on a chlorsis mutant in Brassica juncea L.

In order to reveal the mechanism of chlorsis mutant,the characteristics of chlorisis mutant in Brassica juncea L. was primarily studied by field investigation,determination of chlorophyll content and analysis of zymogram. Compared with the wild type L638-g,the width of cotyledon,plant height,the number of the primary branches and yield per plant of the chlorisis mutantall reduced. The mutant bloomed one day later,but life time reduced by one day,which indicated that the chlorisis mutant had a large influence on plant development. The content of chlorophyll a, chlorophyll b,and carotenoid in the leaves of the mutant plants decreased,especially the content of chlorophyll b decreased sharply,which showed the mutation had influence on the transform action from chlorophyll a to chlorophyll b. No obvious difference of the zymogram of peroxidase and esterase isozyme was observed between the mutant and the wild type. In summary, the chlorisis mutant in Brassica juncea L. probably belonged to one of the total chlorophyll deficient mutants.