| 臭椿病毒病病原鉴定 |
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| 引用本文： | 牛颜冰,姚敏,王德富,王贵军,弓强. 臭椿病毒病病原鉴定[J]. 植物病理学报, 2011, 41(4): 437-440 |
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| 作者姓名： | 牛颜冰 姚敏 王德富 王贵军 弓强 |
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| 作者单位： | 山西农业大学生命科学学院, 太谷030801 |
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| 基金项目： | 国家自然科学基金项目(30571216); 山西省自然科学基金资助项目(20051071) |
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| 摘 要： | 臭椿(Ailanthus altissima)属苦木科(Sima-roubaceae)落叶乔木,为我国常用中草药;以根皮入药,具有消炎、杀菌、杀虫、抗肿瘤及抗病毒等作用;亦可用在建筑、能源、环保和造纸等方面,具有广阔的开发和应用前景。已有研究表明,引起臭椿花叶症状的病毒病原主要
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| 关 键 词： | 病毒病原 烟草花叶病毒 应用前景 病原鉴定 中草药 落叶乔木 抗肿瘤 病毒分离 黄瓜花叶病毒 臭椿 |
| 收稿时间： | 2012-01-06; |
Identification of the pathogenic virus on Ailanthus altissima |
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| NIU Yan-bing,YAO Min,WANG De-fu,WANG Gui-jun,GONG Qiang. Identification of the pathogenic virus on Ailanthus altissima[J]. Acta Phytopathologica Sinica, 2011, 41(4): 437-440 |
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| Authors: | NIU Yan-bing YAO Min WANG De-fu WANG Gui-jun GONG Qiang |
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| Affiliation: | College of Life Sciences, Shanxi Agricultural University,Taigu 030801, China |
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| Abstract: | Leaves from Ailanthus altissima showing severe symptoms were selected and indexed by antigen-coated plate (ACP)-ELISA. Tobacco mosaic virus (TMV) was found in all the samples tested. By using dsRNA as template, one-step RT-PCR was performed to confirm the presence of TMV with specific primers designed for the partial movement protein gene (MP), and a fragment of about 470 bp(GenBank GU167975)was amplified. Sequence analysis demonstrated that Ail isolate showed 75.5%～99.6% identity with other TMV isolates, and shared the highest identities of 99.6% at nucleotide (nt) level with isolate B8 from Spain. From the above, it can be concluded that the virus disease pathogen infecting Ailanthus altissima was TMV. |
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| Keywords: | Ailanthus altissima ACP-ELISA dsRNA RT-PCR sequence analysis |
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