| 顶果木组培增殖培养基的优化 |
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| 引用本文： | 徐位力,许潮漩,林荣华,符晓萍.顶果木组培增殖培养基的优化[J].广东林业科技,2014,30(4):58-61. |
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| 作者姓名： | 徐位力 许潮漩 林荣华 符晓萍 |
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| 作者单位： | 广州市林业科学研究所景观建设有限公司,广州市增城林场,广州市增城林场,广州市林业科学研究所景观建设有限公司 |
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| 基金项目： | 广东省林业科技创新专项“顶果木引种和高效栽培技术研究与示范”（2010KJCX005） |
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| 摘 要： | 采用二次正交旋转组合设计对顶果木（Acrocarpus fraxinifolius）增殖培养基进行优化,建立增殖倍数（Y）对培养基中Ca2＋浓度（X1）、6-BA浓度（X2）及IBA浓度（X3）的正交回归模型：Y=2.416-0.172X1-0.255X2＋0.183X12-0.211X22 ＋0.162X32 ＋0.331X1X2.从模型可知,当Ca2＋浓度为255 mg/L,6-BA浓度为0.76 mg/L,IBA浓度为0.16 mg/L时,增殖倍数达最大值4.45.模型预测值与实际验证结果较接近.
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| 关 键 词： | 顶果木 组织培养 增殖 二次正交旋转组合设计 |
| 收稿时间： | 2014/3/11 0:00:00 |
| 修稿时间： | 2014/5/17 0:00:00 |
Study on Optimal Multiplication Medium of Acrocarpus fraxinifolius |
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| XU Weili,XU Chaoxuan,LIN Ronghua and FU Xiaoping.Study on Optimal Multiplication Medium of Acrocarpus fraxinifolius[J].Forestry Science and Technology of Guangdong Province,2014,30(4):58-61. |
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| Authors: | XU Weili XU Chaoxuan LIN Ronghua and FU Xiaoping |
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| Institution: | XU Weili,XU Chaoxuan,LIN Ronghua,FU Xiaoping,MA Wenqing,LAI Shuxiong(Landscape construction Co., Ltd.of Guangzhou Forestry Research Institute, Guangzhou, Guangdong 510515, China;Zengcheng Forest Farm of Guangzhou City, Guangzhou, Guangdong 511300, China) |
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| Abstract: | The multiplication medium of Acrocarpus fraxinifolius was optimized with quadratic orthogonal rotation combination design.The quadratic orthogonal regression model of multiplication rate （Y） to three factors including concentration of Ca2＋ （X1）,6-BA（X2） and IBA（X3） was established as Y =2.420-0.172X1-0.255X2 ＋ 0.184X12-0.211X22 ＋ 0.162X32 ＋ 0.331X1X2.It was concluded from the model that when the concentration of Ca2＋ （X1）,6-BA（X2） and IBA（X3） was 255 mg/L,0.76 mg/L and 0.16 mg/L respectively,the r axirum rultiplication rate （Y） was 4.45 which was close to the experiment result. |
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| Keywords: | Acrocarpus fraxinifolius tissue culture proliferation quadratic orthogonal rotation combination design |
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