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Comparison of mycoplasma ovipneumoniae isolates using bacterial restriction endonuclease DNA analysis and SDS-PAGE |
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| Authors: | Alison J. Mew G. Ionas J.K. Clarke A.J. Robinson R.B. Marshall |
| Affiliation: | a Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand b Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand |
| Abstract: | Sixteen isolates of Mycoplasma ovipneumoniae recovered from the nasal tract or lungs of sheep from different flocks in New Zealand were examined by bacterial restriction endonuclease DNA analysis (BRENDA) using EcoR1 and by sodium dodecyl sulphate-polycrylamide gel electrophoresis (SDS-PAGE). All isolates gave BRENDA patterns which differed entirely from one another. Following 20 serial passages (corresponding to approximately 67 generations) of an isolate, no charge was detected in the BRENDA pattern. When eight isolates were examined by SDS-PAGE most bands were common but, nevertheless, each isolate was unique in the sense that they differed from one another in one or more bands. The marked heterogeneity of patterns observed when strains of M. ovipneumoniae are compared by BRENDA, together with the stability of such patterns over many generations, will enable this approach to be used to study the epidemiology of individual strains of M. ovipneumoniae with a flock. |
| Keywords: | To whom requests for reprints should be directed. |
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