冷应激对海兰雏鸡某些血相变化的影响

采用100只雄性海兰雏鸡进行急性（24h以内）、慢性冷应激（低5℃）及冷适应实验，通过瑞氏染色和脂酶染色法观察外周血白细胞分类及T淋巴细胞数目的变化。结果表明：急性冷应激期间，嗜碱性细胞数目增加，异嗜性细胞／淋巴细胞（H／L）明显增加，应激24h后，淋巴细胞明显减少（P＜0．05），冷适应后淋巴细胞数目显著增加（P＜0．01）、T淋巴细胞数目极显著增加（P＜0．001）、H／L明显减少（P＜0．01）。结果提示低5℃冷应激使雏鸡细胞免疫水平增加。冷应激15d后雏鸡已经开始适应。

The Influence of Cold Stress on Some Parameters of Blood in Hailan Chickens

In cold stress experiment，100 male HaiLan chickensfed at relatively cold condition（lower 5 ℃ than normal temperature） were used to observe changes in amount of white blood cells classification and T-lymphocyte stained by Wrig hts dye and ANAE. The results showed as follows: during acute cold stress, the number of basophil, heterophil/lymphocyte(H/L) ratio were increased significantly （ P ＜0.05）; After cold acclimatization, H/L ratio was markedly lower than that in contr ol（P ＜ 0.01）, and the numbers of lymphocyte and T-cells were increased s ignif icantly（P ＜ 0.01）. The results indicated that the level of cellular immun ity i n chickens was improved during cold stress, After 15 days, chickens had adapted relatively cold environment（lower 5 ℃ than normal temperature）.