Transmission of mitochondrial plasmids in protoplast cell fusion between compatible monokaryons of <Emphasis Type="Italic">Lentinula edodes</Emphasis> |
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Authors: | Masaki Fukuda Izumi Sendo Satoko Imahori Yukitaka Fukumasa-Nakai |
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Institution: | (1) Faculty of Agriculture, Shinshu University, Minamiminowa, Nagano 399-4598, Japan;(2) Tottori Mycological Institute, Tottori 689-1125, Japan |
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Abstract: | Electrophoretic analysis of the transmission pattern of mitochondrial plasmids in protoplast cell fusion between compatible
monokaryons of Lentinula edodes indicates that three of the four plasmids carried in parental monokaryons are effectively transferred and replicated in the
protoplast fusants. The two monokaryons, 1158a and 1569a, carried different plasmids that could be distinguished by a single
restriction digest. Electrophoresis of intact plasmids and restriction analyses indicate that all but one of the fusants carry
three of the four possible plasmids, indicating that transmission of plasmids in protoplast fusions is principally biparental
in L. edodes. Thus, heterocytoplasmic cells of L. edodes can be effectively constructed by protoplast cell fusion. In addition, plasmids of the same homology group cannot coexist
in the heteroplasmic cells after protoplast cell fusion.
Contribution No. 382 of the Tottori Mycological Institute |
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Keywords: | Mitochondrial plasmid Protoplast cell fusion Lentinula edodes |
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