Tissue-specific downregulation of the adiponectin “system”: possible implications for fat accumulation tendency in the pig |
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Authors: | A De Rosa ML Monaco E Nigro O Scudiero M D'Andrea F Pilla G Oriani A Daniele |
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Institution: | 1. CEINGE- Biotecnologie Avanzate Scarl, Via Gaetano Salvatore 482, 80145, Napoli, Italy;2. Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Seconda Università degli Studi di Napoli, Via Vivaldi 42, Caserta, Italy;3. IRCCS-Fondazione SDN, Via Gianturco 113, Napoli, Italy;4. Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via Pansini 5, Napoli, Italy;5. Dipartimento di Agricoltura, Ambiente, Alimenti, Università degli Studi del Molise, Via De Sanctis, Campobasso, Italy;6. Dipartimento di Scienze per la Salute, Università degli Studi del Molise, Via De Sanctis, Campobasso, Italy |
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Abstract: | Adiponectin's beneficial effects are mediated by the AdipoR1 and AdipoR2 receptors (AdipoRs). The pig is a good model to study complex disorders such as obesity. We analyzed the expression of adiponectin, AdipoRs and some key molecules of energy metabolism (AMP-activated protein kinase α AMPKα], p38 mitogen-activated protein kinase p38 MAPK], and PPARα) in 2 pig breeds that displayed an opposite genetic behavior for energy metabolism: Casertana (CE), a fat-type animal, and Large White (LW), a lean-type animal. Muscle, liver, visceral and subcutaneous adipose tissues, and brain tissues were examined. The AdipoRs cDNA sequences were identical in the 2 breeds. AdipoRs mRNA expression, measured in all tissues, was significantly lower only in the 2 adipose tissues of CE pigs (P < 0.05). The muscle expression of AdipoRs, AMPKα, p38 MAPK, and PPARα was lower in CE than in LW animals (P < 0.01, P < 0.05, P < 0.01, P < 0.01, respectively). In liver, no molecule differed between breeds. The expression of both AdipoRs in visceral and subcutaneous adipose tissues was lower in CE pigs (P < 0.01). In brain, AdipoR1 and AMPKα expression was lower in CE pigs (P < 0.01), whereas AdipoR2 tended to be lower in CE than LW pigs (P = 0.05). In conclusion, our results suggest that tissue-specific downregulation of Adiponectin, AdipoRs, and of the key molecules of energy metabolism may be associated with the tendency of CE pigs to accumulate fat. |
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Keywords: | Adiponectin AdipoR1 AdipoR2 Energy metabolism Pig Fat accumulation |
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