| 鸡源和鸭源减蛋综合征病毒DNA酶切图谱分析 |
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| 引用本文： | 周锦萍,李刚,郑明球,蔡宝祥.鸡源和鸭源减蛋综合征病毒DNA酶切图谱分析[J].南京农业大学学报,1999,22(2):71-75. |
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| 作者姓名： | 周锦萍 李刚 郑明球 蔡宝祥 |
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| 作者单位： | 南京农业大学农业部动物疫病诊断与免疫重点开放实验室 |
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| 摘 要： | 采用差速离心法纯化鸡源和鸭源减蛋综合征（ＥＤＳ）病毒，酚－氯仿抽提法提取两种毒株的核酸。选取ＥｃｏＲⅠ、ＢａｍＨⅠ、ＢｇｌⅠ、ＢｇｌⅡ、ＫｐｎⅠ、ＰｓｔⅠ和ＨｉｎｄⅢ７种限制性内切酶，对两种毒株进行酶切图谱比较。结果显示，鸡源株酶切片段数分别为４，４，６，８，５，９，１０，共计４６个片段；鸭源株产生的酶切片段数分别为４，４，５，７，９，１０，８，共计４７个片段。两者的酶切结果除ＥｃｏＲⅠ完全相同外，其余均存在不同程度的差异。由此表明，鸡源和鸭源ＥＤＳ病毒虽然血清型相同，但在基因水平上存在较大的差异，属不同基因型。
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| 关 键 词： | EDS病毒 病毒核酸 酶切图谱 |
| 修稿时间： | 1998-06-26 |
Restriction endonuclease analysis of egg drop syndrome virus |
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| Zhou Jinping,Li Gang,Zheng Mingqiu and Cai Baoxiang.Restriction endonuclease analysis of egg drop syndrome virus[J].Journal of Nanjing Agricultural University,1999,22(2):71-75. |
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| Authors: | Zhou Jinping Li Gang Zheng Mingqiu and Cai Baoxiang |
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| Abstract: | By using differential centrifugation,EDSV isolates from chicken (NE4 strain)and duck(JE1 strain)were purified.Purified virus was treated with proteinase K and extracted with phenol,then restriction endonuclease maps of two strains were compared by using seven different restriction endonucleases of EcoRBamHBglBglKpnPst and Hin d.It was found that NE4 showed 4,4,6,8,5,9,10 framents and JE1 showed 4,4,5,7,9,10,8 fragments.Between NE4 and JE1 only EcoR s maps were same,other endonucleases maps were apparently different in patterns and size of fragments.It suggested that EDSV strains from different hosts with same serotype had different genomy. |
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| Keywords: | EDSV DNA restriction map |
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