| 栽培稻与Oryza officinalis相似群野生稻基因组VDAC基因的比较 |
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| 引用本文： | 马亢,周杰,覃瑞,刘新琼,刘学群,王春台.栽培稻与Oryza officinalis相似群野生稻基因组VDAC基因的比较[J].华中农业大学学报,2008,27(3). |
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| 作者姓名： | 马亢 周杰 覃瑞 刘新琼 刘学群 王春台 |
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| 作者单位： | 中南民族大学生命科学学院/生物技术国家民委重点实验室,武汉,430074 |
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| 基金项目： | 国家自然科学基金
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国家高技术研究发展计划(863计划)
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教育部留学回国人员科研启动基金
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中南民族大学校科研和教改项目 |
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| 摘 要： | 根据水稻基因组文库日本晴的基因组VDAC基因的序列,设计引物分别扩增6种不同基因组的野生稻(BB、CC、BBCC及CCDD)和2种栽培稻(AA)的基因组DNA的VDAC基因片段.所有分别代表8个VDAC基因的8对引物中,除引物VDAC3外,其它7对引物均能扩增出预期大小的特异条带,其中一些片段是所有试验材料共有的,而另一些则具有明显的基因组或种的特异性.AA、BB、CC基因组均具有VDAC1、VDAC4、VDAC7和VDAC8引物的扩增位点,而DD基因组则不能确定.VDAC6的引物位点是AA基因组所特有.VDAC2引物的扩增位点存在于基因组AA、BB、DD,而CC基因组中则无此位点.而VDAC5则可区分同为CCDD的宽叶野生稻和高秆野生稻.栽培稻种与野生稻种基因组相比能扩增出更多的VDAC基因片段的试验结果,为进一步研究稻属中VDAC基因的起源及进化关系提供了基础.
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| 关 键 词： | 栽培稻 野生稻 VDAC基因 |
Comparison of VDAC Genes between Cultivated and Oryza officinalis Group Wild Rice in Genome DNA |
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| MA Kang ZHOU Jie QIN Rui LIU Xin-qiong LIU Xue-qun WANG Chun-tai.Comparison of VDAC Genes between Cultivated and Oryza officinalis Group Wild Rice in Genome DNA[J].Journal of Huazhong Agricultural University,2008,27(3). |
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| Authors: | MA Kang ZHOU Jie QIN Rui LIU Xin-qiong LIU Xue-qun WANG Chun-tai |
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| Institution: | College of Life Science/Key Lab.for Biotechnology of the State EthnicAffairs Commission;South-Central University for Nationalities;Wuhan 430074;China |
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| Abstract: | According to the sequence from the rice date base,8 primer pairs for 8 VDAC genes were designed.The fragments of 8 VDAC genes were amplified with the template DNA of different wild rice(with BB,CC,BBCC and CCDD genome) and cultivated rice(AA genome) through the VDAC 1-8 primers.Within the 8 primers,there were 7 primer pairs that special bands were amplified.The results showed that some VDAC fragments appeared in all studied materials,and some represent the difference between the different genomes or species... |
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| Keywords: | cultivated rice wild rice VDAC gene |
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