|
|||||
|
|
| 黄瓜再生体系和除草剂抗性筛选体系的建立 | |
| 引用本文： | 刘冰,林毓娥,黄河勋,李玲,王瑞.黄瓜再生体系和除草剂抗性筛选体系的建立[J].中国瓜菜,2012,25(3):37-39. |
| 作者姓名： | 刘冰 林毓娥 黄河勋 李玲 王瑞 |
| 作者单位： | 1. 广东省农科院蔬菜研究所·广东省蔬菜新技术重点实验室 广州 510640;华南师范大学生命科学学院·广东省植物发育生物工程重点实验室 广州 510631 2. 广东省农科院蔬菜研究所·广东省蔬菜新技术重点实验室 广州 510640 3. 华南师范大学生命科学学院·广东省植物发育生物工程重点实验室 广州 510631 |
| 基金项目： | 广东省自然科学基金项目(10151064001000001) |
| 摘 要： | 试验以黄瓜自交系C-8子叶节为材料,比较了不同浓度的6-BA和IAA组合对其不定芽诱导的影响,建立了高效快速的再生体系;摸索了不同浓度的草铵膦对产生不定芽的影响,建立了C-8的草铵膦抗性筛选体系,为下一步的遗传转化打下基础。结果表明,最佳的诱芽培养基为MS+0.5mg·L-16-BA+2mg·L-1IAA,出芽率为77.5%,生根培养基为1/2MS。对于C-8黄瓜,草铵膦的最佳遗传转化筛选浓度为0.6mg·L-1。 |
| 关 键 词： | 黄瓜 再生体系 草铵膦 |
Establishment of Genetic Transformation and Herbicide Tolerance Screening System in Cucumber |
|
| LIU Bing , LIN Yu-e , HUANG He-xun , LI Ling , WANG Rui.Establishment of Genetic Transformation and Herbicide Tolerance Screening System in Cucumber[J].China Cucurbits And Vegetables,2012,25(3):37-39. | |
| Authors: | LIU Bing LIN Yu-e HUANG He-xun LI Ling WANG Rui |
| Institution: | 1 (1. Vegetables Research Institute, Academy of Agricultural Sciences, Guangzhou, Guangdong, 510640, China; 2. Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou, Guangdong, 510631, China) |
| Abstract: | We studied the effect of different concentration combinations of 6-BA and IAA on in vitro shoot organogenesis and cucumber plant regeneration by using cotyledon tissue of cucumber inbred line C-8. A high frequency regeneration system was established. In addition, we researched the Glufosinate-ammoniu tolerance screening system for cucumber line C-8. The C-8 cotyledon node explants regenerated plantlets well in MS medium+0.5 mg·L-1 6-BA+2 mg·L-1 IAA with 77.5% regeneration rate. The regenerated shoots rooted well in 1/2 MS medium. For genetic transformation of cucumber line C-8, the best herbicide tolerance screening concentration is Glufosinate-ammoniu at 0.6 mg·L-1. |
| Keywords: | Cucumber Regeneration system Glufosinate-ammonium |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |