Somatic embryogenesis inCephalotaxus harringtonia embryo-megagametophyte co-culture |
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Authors: | René Rohr Florence Piola Patricia Pasquier |
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Institution: | (1) Interactions Plantes-Champignons et Micropropagation, Bat. 405, Université de Lyon 1, 69622 Villeurbanne Cedex, France |
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Abstract: | Somatic embryogenesis was initiated fromCephalotaxus harringtonia (Forbes) K. Koch embryo culture. Explants consisted of embryo and megagametophyte halves both cut longitudinally. They were
removed aseptically from mature seeds and grown together on a solid Murashige and Skoog modified medium supplemented with
5 mg·l
−1 2,4-dichlorophenoxyacetic acid. Embryogenic cultures started from callus after three or more months on the primary medium.
The embryogenic callus originated from the suspensor region of the embryo. All chromosome counts made in the cells of the
embryonic structures demonstrated a diploid stage, which suggest that they originated from zygotic embryo tissue. The early
stages of somatic embryogenic development were achieved,i.e., formation of small clusters consisting of an embryonal region made up of isodiametric meristematic cells. A more advanced
stage was reached in some cultures in which the distal embryonal end of the embryo appeared smooth and opaque. The ultrastructural
characteristics of the embryos, the two types of embryo cells, embryonal and suspensor cells, as well as their contents were
similar to those already reported in the case of somatic embryogenesis of other conifers. |
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Keywords: | Cephalotaxus embryogenic callus somatic embryogenesis ultrastructure |
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